Maternal T Cell Engraftment in SCID
Ordering Recommendation
Use to monitor engraftment of maternal T cells in patients with severe combined immunodeficiency (SCID) prior to allogenic stem cell transplantation.
New York DOH Approval Status
Specimen Required
Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A).
New York State Clients: Lavender (EDTA)
Transport 3 mL whole blood. (Min: 2 mL)
New York State Clients: Transport 8 mL whole blood. (Min: 4 mL). Specimen must be received at performing laboratory within 24 hours of collection. For specimen requirements and direct submission instructions please contact ARUP Referral Testing at 800-242-2787 ext. 5145.
Ambient. Also acceptable: refrigerated. Ship overnight. Specimens should be received within 24 hours of collection for optimal isolation of T cells.
New York State Clients: Ambient
Clotted or hemolyzed specimens.
Room temperature: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: Room Temperature: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Methodology
Polymerase Chain Reaction (PCR)/Fragment Analysis
Performed
Sun-Sat
Reported
5-9 days
Reference Interval
Type Maternal | Maternal cells only. |
Type Patient | Patient cells only. |
Mixed | Patient and maternal T cells present. Semiquantitative results of percentage of patient and maternal cells will be reported. |
Interpretive Data
Refer to report.
Laboratory Developed Test (LDT)
Note
To complete Maternal T Cell Engraftment in SCID testing, samples should be collected to perform the following three tests: (1) A buccal swab or brush collected from the patient for Maternal T Cell Engraftment in SCID, Pre-Engraftment Specimen (ARUP test code 2014694), used as a genetic baseline for the patient. (2) A peripheral blood sample from the biological mother for Maternal T Cell Engraftment in SCID, Maternal Specimen (ARUP test code 2014704), used as a genetic baseline for the mother. (3) A peripheral blood sample collected from the patient for Maternal T Cell Engraftment in SCID (ARUP test code 2014699). T cells isolated from the blood sample will be genotyped for comparison to the patient and biological mother baseline genotypes. If T-cell sorting is not completed on the blood sample before submission of Maternal T Cell Engraftment in SCID (ARUP test code 2014699), BMT Cell Isolation (ARUP test code 2005498) will be added to each order of Maternal T Cell Engraftment in SCID (ARUP test code 20146990). Additional charges apply for cell isolation.
Hotline History
CPT Codes
81268; If cell sorting is performed, add 88184
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2014700 | Specimen, Maternal Engraftment | |
2014701 | Mat Engraftment, Informative Loci | |
2014702 | Maternal Engraftment, Interpretation |
Aliases
- Maternal engraftment
- SCID engraftment