Time Sensitive

Ordering Recommendation

Use to monitor engraftment of maternal T cells in patients with severe combined immunodeficiency (SCID) prior to allogenic stem cell transplantation.

New York DOH Approval Status

Testing is not New York state approved. Specimens from New York clients will be sent out to a New York state-approved laboratory.

Specimen Required

Patient Preparation

Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A).
New York State Clients: Lavender (EDTA)

Specimen Preparation

Transport 3 mL whole blood. (Min: 2 mL)
New York State Clients: Transport 8 mL whole blood. (Min: 4 mL). Specimen must be received at performing laboratory within 24 hours of collection. For specimen requirements and direct submission instructions please contact ARUP Referral Testing at 800-242-2787 ext. 5145.

Storage/Transport Temperature

Ambient. Also acceptable: refrigerated. Ship overnight. Specimens should be received within 24 hours of collection for optimal isolation of T cells.
New York State Clients: Ambient

Unacceptable Conditions

Clotted or hemolyzed specimens.


Room temperature: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable
New York State Clients: Room Temperature: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable


Polymerase Chain Reaction (PCR)/Fragment Analysis




5-9 days

Reference Interval

Type Maternal Maternal cells only.
Type Patient Patient cells only.
Mixed Patient and maternal T cells present. Semiquantitative results of percentage of patient and maternal cells will be reported.

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)


To complete Maternal T Cell Engraftment in SCID testing, samples should be collected to perform the following three tests: (1) A buccal swab or brush collected from the patient for Maternal T Cell Engraftment in SCID, Pre-Engraftment Specimen (ARUP test code 2014694), used as a genetic baseline for the patient. (2) A peripheral blood sample from the biological mother for Maternal T Cell Engraftment in SCID, Maternal Specimen (ARUP test code 2014704), used as a genetic baseline for the mother. (3) A peripheral blood sample collected from the patient for Maternal T Cell Engraftment in SCID (ARUP test code 2014699). T cells isolated from the blood sample will be genotyped for comparison to the patient and biological mother baseline genotypes. If T-cell sorting is not completed on the blood sample before submission of Maternal T Cell Engraftment in SCID (ARUP test code 2014699), BMT Cell Isolation (ARUP test code 2005498) will be added to each order of Maternal T Cell Engraftment in SCID (ARUP test code 20146990). Additional charges apply for cell isolation.

Hotline History


CPT Codes

81268; If cell sorting is performed, add 88184


Component Test Code* Component Chart Name LOINC
2014700 Specimen, Maternal Engraftment
2014701 Mat Engraftment, Informative Loci
2014702 Maternal Engraftment, Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Maternal engraftment
  • SCID engraftment
Maternal T Cell Engraftment in SCID