Monitor engraftment of maternal T cells in patients with severe combined immunodeficiency (SCID) prior to allogenic stem cell transplantation.
Polymerase Chain Reaction/Fragment Analysis
Buccal swab or buccal brush.
Collect 3-4 swab/brush samples from patient and place in dry, sterile container for transport.
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Refer to report.
Laboratory Developed Test (LDT)
To complete Maternal T Cell Engraftment in SCID testing, samples should be collected to perform the following three tests: (1) A buccal swab or brush collected from the patient for Maternal T Cell Engraftment in SCID, Pre-Engraftment Specimen (ARUP test code 2014694), used as a genetic baseline for the patient. (2) A peripheral blood sample from the biological mother for Maternal T Cell Engraftment in SCID, Maternal Specimen (ARUP test code 2014704), used as a genetic baseline for the mother. (3) A peripheral blood sample collected from the patient for Maternal T Cell Engraftment in SCID, (ARUP test code 2014699). T cells isolated from the blood sample will be genotyped for comparison to the patient and biological mother baseline genotypes. If T cell sorting is not completed on the blood sample before submission, BMT Cell Isolation (ARUP test code 2005498) will be added. Additional charges apply.
|Component Test Code*||Component Chart Name||LOINC|
|2014695||Specimen, Maternal Engraftment Pre|
|2014696||Maternal Engraftment, Pre Interp|
|2014697||Mat Engraftment, Pre, Informative Loci|
- Maternal cell engraftment
- Maternal engraftment
- SCID engraftment