Ordering Recommendation

Order before initiating treatment with NS5A inhibitors. Use to determine HCV type 1-6 after molecular confirmation of positive HCV screen. If genotype “1a or 1b” is determined, testing will reflex to HCV NS5A for genotype differentiation and drug resistance by sequencing.

Mnemonic

HCV RFX 5A

Methodology

Polymerase Chain Reaction/Sequencing

Performed

Sun-Sat

Reported

13-19 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Lavender (EDTA), Pink (K2EDTA), Plasma Preparation Tube (PPT), or Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1.5 mL)

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

Heparinized specimens.

Remarks
Stability

Ambient: Unacceptable; Refrigerated: 72 hours; Frozen: 4 months

Reference Interval

By report

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

This test may be unsuccessful if the HCV RNA viral load is less than log 3.6 or 4000 IU/mL. If initial result is Type "1a or 1b", then HCV NS5A Drug Resistance by Sequencing will be added. Additional charges apply.

Hotline History

N/A

CPT Codes

87902; if reflexed, add 87902

Components

Component Test Code* Component Chart Name LOINC
0055593 HCV Genotype by Sequencing 32286-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Hepatitis C Virus (HCV) Genotype with Reflex to HCV NS5A Drug Resistance by Sequencing