Search our extensive Laboratory Test Directory to find test codes, ordering recommendations, specimen stability information, Test Fact Sheets, and more.
Recommendations when to order or not order the test. May include related or preferred tests.
Support the diagnosis of autoimmune lymphoproliferative syndrome (ALPS).
New York DOH Approval Status
Indicates whether a test has been approved by the New York State Department of Health.
Testing is not New York state approved. Specimens from New York clients will be sent out to a New York state-approved laboratory.
Specimen Required
Patient PreparationInstructions patient must follow before/during specimen collection.
CollectSpecimen type to collect. May include collection media, tubes, kits, etc.
Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA) New York State Clients: Lavender (EDTA) or pink (K2EDTA)
Specimen PreparationInstructions for specimen prep before/after collection and prior to transport.
Transport 5 mL whole blood. (Min: 0.5 mL) New York State Clients: Specimen must be received at performing laboratory within 72 hours. Do not send to ARUP Laboratories. For direct submission instructions please contact ARUP Referral Testing at 800-242-2787 ext. 5161.
Storage/Transport TemperaturePreferred temperatures for storage prior to and during shipping to ARUP. See Stability for additional info.
CRITICAL ROOM TEMPERATURE
Unacceptable ConditionsCommon conditions under which a specimen will be rejected.
Clotted or hemolyzed specimens
RemarksAdditional specimen collection, transport, or test submission information.
Specimens must be analyzed within 48 hours of collection
StabilityAcceptable times/temperatures for specimens. Times include storage and transport time to ARUP.
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable New York State Clients: Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Methodology
Process(es) used to perform the test.
Quantitative Flow Cytometry
Performed
Days of the week the test is performed.
Sun-Sat
Reported
Expected turnaround time for a result, beginning when ARUP has received the specimen.
1-3 days
Reference Interval
Normal range/expected value(s) for a specific disease state. May also include abnormal ranges.
Test Number
Components
Reference Interval
Absolute alpha/beta TCR+ DNT
Age
Reference Interval (cells/uL)
2-18 years old
0-46
18-69 years old
0-32
Absolute alpha/beta TCR+ DNT B220+
Age
Reference Interval (cells/uL)
2-18 years old
0-5
18-69 years old
0-6
% alpha/beta TCR+ DNT
Age
Reference Interval (%)
2-18 years old
0-3
18-69 years old
0-2
% alpha/beta TCR+ DNT B220+
Age
Reference Interval (%)
2-18 years old
0-0.3
18-69 years old
0-0.4
Interpretive Data
May include disease information, patient result explanation, recommendations, or details of testing.
Reported percentages reflect percentage of CD3 positive lymphocytes. The hallmark for a diagnosis of autoimmune lymphoproliferative syndrome (ALPS) is an increased concentration of CD3+ T-cells negative for CD4 and CD8 (double-negative T-cells [DNT]) and positive for the alpha/beta T-cell receptor (TCR). B220 expression on alpha/beta TCR+DNT cells is a sensitive and specific marker for ALPS and is associated with mutations in the FAS gene.
Abnormal results should be correlated with clinical history and confirmed by additional testing for defective in vitro lymphocyte apoptosis and for mutations in the FAS gene.
Compliance Category
Laboratory Developed Test (LDT)
Note
Additional information related to the test.
Hotline History
N/A
CPT Codes
The American Medical Association Current Procedural Terminology (CPT) codes published in ARUP's Laboratory Test Directory are provided for informational purposes only. The codes reflect our interpretation of CPT coding requirements based upon AMA guidelines published annually. CPT codes are provided only as guidance to assist clients with billing. ARUP strongly recommends that clients confirm CPT codes with their Medicare administrative contractor, as requirements may differ. CPT coding is the sole responsibility of the billing party. ARUP Laboratories assumes no responsibility for billing errors due to reliance on the CPT codes published.
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
Other names that describe the test. Synonyms.
Alpha Beta Double Negative T-Cells for Autoimmune Lymphoproliferative Syndrome
Connect Sign In
