Antimicrobial Susceptibility - Surveillance Carbapenemase Gene Detection by PCR
Detect the presence of predominant carbapenemase genes (blaKPC, blaNDM, blaVIM, blaOXA-48, blaIMP) responsible for resistance to carbapenem and other beta-lactam antibiotics. Test is performed on rectal swab specimen.
Qualitative Polymerase Chain Reaction
New York DOH Approval Status
Rectal eSwab. Refer to collection instructions at https://www.aruplab.com/Specimen-Handling/resources/pdf/rectal-eswab.pdf
Transport rectal swab in eSwab transport media (ARUP Supply #45877) available online through eSupply using ARUP ConnectTM or contact ARUP Client Services at (800) 522-2787. Place each specimen in an individually sealed bag.
Refrigerated. Also acceptable: Frozen.
Specimen source required.
Ambient: 48 hours; Refrigerated: 6 days; Frozen: 6 days
This assay detects five carbapenemase gene families (blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP) encoding enzymes that may confer resistance to carbapenem and other beta-lactam antibiotics. This assay is intended for use as an aid to infection control in the detection of carbapenem-resistant bacteria and is not intended to guide or monitor treatment of infection. A negative result does not exclude the presence of other resistance mechanisms or assay-specific nucleic acid in concentrations below the level of detection.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
This assay will generate a negative IMP result when testing samples containing IMP-7, IMP-13 or IMP-14 gene sequences, and may detect IMP-4 at reduced sensitivity. False-negative results may be encountered in rectal specimens with Pseudomonas aeruginosa containing the blaVIM gene and with Acinetobacter baumanii containing blaIMP gene.
|Component Test Code*||Component Chart Name||LOINC|
|2014278||KPC gene by PCR||49617-4|
|2014279||NDM gene by PCR||73982-1|
|2014280||OXA-48 gene by PCR||63368-5|
|2014281||VIM gene by PCR||63368-5|
|2014282||IMP gene by PCR||63368-5|
- Klebsiella pneumoniae carbapenemase
- multi-drug resistance
- NewDelhi metallo-B-lactamase
- resistance mechanism