Antimicrobial Susceptibility - Surveillance Carbapenemase Gene Detection by PCR
Detect the presence of predominant carbapenemase genes (blaKPC, blaNDM, blaVIM, blaOXA-48, blaIMP) responsible for resistance to carbapenem and other beta-lactam antibiotics. Test is performed on rectal swab specimen.
Qualitative Polymerase Chain Reaction
Rectal eSwab. Refer to collection instructions at https://www.aruplab.com/Specimen-Handling/resources/pdf/rectal-eswab.pdf
Transport rectal swab in eSwab transport media (ARUP Supply #45877) available online through eSupply using ARUP ConnectTM or contact ARUP Client Services at (800) 522-2787. Place each specimen in an individually sealed bag.
Refrigerated. Also acceptable: Frozen.
Specimen source required.
Ambient: 48 hours; Refrigerated: 6 days; Frozen: 6 days
This assay detects five carbapenemase gene families (blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP) encoding enzymes that may confer resistance to carbapenem and other beta-lactam antibiotics. This assay is intended for use as an aid to infection control in the detection of carbapenem-resistant bacteria and is not intended to guide or monitor treatment of infection. A negative result does not exclude the presence of other resistance mechanisms or assay-specific nucleic acid in concentrations below the level of detection.
This assay will generate a negative IMP result when testing samples containing IMP-7, IMP-13 or IMP-14 gene sequences, and may detect IMP-4 at reduced sensitivity. False-negative results may be encountered in rectal specimens with Pseudomonas aeruginosa containing the blaVIM gene and with Acinetobacter baumanii containing blaIMP gene.
|Component Test Code*||Component Chart Name||LOINC|
|2014278||KPC gene by PCR||49617-4|
|2014279||NDM gene by PCR||73982-1|
|2014280||OXA-48 gene by PCR||63368-5|
|2014281||VIM gene by PCR||63368-5|
|2014282||IMP gene by PCR||63368-5|
- Klebsiella pneumoniae carbapenemase
- multi-drug resistance
- NewDelhi metallo-B-lactamase
- resistance mechanism