Antimicrobial Susceptibility - Carbapenemase Gene Detection by PCR

2014277
copy
 

Copy Utility


emailprint
Example Reports
Detected
Not Detected
Ordering Recommendation

Detect the presence of predominant carbapenemase genes (blaKPC, blaNDM, blaVIM, blaOXA-48, blaIMP) responsible for resistance to carbapenem and other beta-lactam antibiotics.

Mnemonic
CARBAR PCR
Methodology

Qualitative Polymerase Chain Reaction

Performed

Sun-Sat

Reported

2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Actively growing Enterobacteriaceae, Pseudomonas aeruginosa, or Acinetobacter baumannii in pure culture.

Specimen Preparation

Transport sealed container with pure culture on agar slant/bacterial transport media. Place each specimen in an individually sealed bag.

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Mixed cultures or nonviable organisms.

Remarks

Isolate identification (for cultures) and specimen source required.

Stability

Ambient: 1 week; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval

Not Detected

Interpretive Data

This assay detects five carbapenemase gene families (blaKPC, blaNDM, blaOXA-48, blaVIM, blaIMP) encoding enzymes that may confer resistance to carbapenem and other beta-lactam antibiotics. This assay is intended for use as an aid to infection control in the detection of carbapenem-resistant bacteria and is not intended to guide or monitor treatment of infection. A negative result does not exclude the presence of other resistance mechanisms or assay-specific nucleic acid in concentrations below the level of detection.

Compliance Category

FDA

Note

An additional processing fee will be billed for all isolates not submitted in pure culture, as indicated in the specimen requirements.

If species identification is not provided, identification will be performed at ARUP. Additional charges apply.

This assay will generate a negative IMP result when testing samples containing IMP-7, IMP-13 or IMP-14 gene sequences, and may detect IMP-4 at reduced sensitivity.

Hotline History
N/A
CPT Codes

87150

Are you an ARUP Client? Click here for your pricing.
Components
Component Test Code* Component Chart Name LOINC
2014278 KPC gene by PCR 49617-4
2014279 NDM gene by PCR 73982-1
2014280 OXA-48 gene by PCR 63368-5
2014281 VIM gene by PCR 63368-5
2014282 IMP gene by PCR 63368-5
2014283 CarbaR Interpretation 48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • carbapenem-resistance
  • carbapenemase
  • CRE
  • Enterobacteriaceae
  • IMP
  • Klebsiella pneumoniae carbapenemase
  • KPC
  • KPCPCR
  • MA KPCPCR
  • MDR
  • multi-drug resistance
  • NDM
  • NewDelhi metallo-B-lactamase
  • OXA-48
  • resistance mechanism
  • VIM
Antimicrobial Susceptibility - Carbapenemase Gene Detection by PCR