IDH1 and IDH2 Mutation Analysis, Exon 4, Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue
IDH1/IDH2 mutational status is a prognostic marker in individuals with low- and high-grade gliomas. Aid in distinguishing a primary from a secondary glioblastoma.
Polymerase Chain Reaction/Sequencing
DNA isolation: Sun-Sat
Tumor tissue. Also acceptable: DNA extracted by CLIA certified lab with corresponding client-circled H&E slide.
Tumor Tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Tissue block will be returned after testing.Transport tissue block or 4 unstained 5-micron slides. (Min: 3 slides) Transport block and/or slide(s) in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Extracted DNA: Refrigerated.
Less than 25 percent tumor. DNA extracted by a non-CLIA lab. DNA extracted without a corresponding circled H&E slide. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens.
For FFPE specimens include surgical pathology report.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Extracted DNA Ambient: 1 month; Refrigerated: Indefinitely; Frozen: Indefinitely
Refer to report.
Laboratory Developed Test (LDT)
88381; 81120; 81121
|Component Test Code*||Component Chart Name||LOINC|
|2006445||IDH1 and IDH2 Mutation Results||35474-6|
|2014216||IDH1-2 FFPE Source|
- IDH1 SNP
- Isocitrate dehydrogenase (IDH) 1 and 2
- Isocitrate Dehydrogenase 1
- Isocitrate Dehydrogenase 2