May be useful in immunization/vaccination trials or may aid in the clinical diagnosis of diseases strongly associated with the HLA-DQ locus. To rule out celiac disease (when other testing is inconclusive), refer to Celiac Disease (HLA-DQ2 and HLA-DQ8) Genotyping (2005018). When narcolepsy is suspected in symptomatic individuals, refer to Narcolepsy (HLA-DQB1*06:02) Genotyping (2005023).
Polymerase Chain Reaction/Sequence-Specific Oligonucleotide Probe Hybridization
Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B).
Transport 5 mL whole blood. (Min: 3 mL)
Green (sodium or lithium heparin) tubes.
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Background Information for HLA-DQ Genotyping:
Purpose: For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-DQ locus.
Methodology: PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-DQ locus.
Analytical Sensitivity & Specificity: Medium to high resolution of HLA-DQ locus.
Limitations: The presence of a disease-associated HLA combination does not establish a diagnosis. If fewer than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation; for pre-transplant allele matching, consider HLA Class I and II Panel (HLA A, HLA B, HLA C, DRB1, DQA1, DQB1, DPB1) by Next Generation Sequencing (ARUP test code 3002061) or HLA Class I and II Panel (HLA A, HLA B, HLA C, DRB1, DRB345, DQA1, DQB1, DPA1, DPB1) by Next Generation Sequencing (ARUP test code 3002062).
Occasionally the specific allele cannot be determined; in this case, the most likely allele assignment is made followed by a sequence of letters indicating other possible allele assignments. Interpretation of allele codes can be found at https://bioinformatics.bethematchclinical.org/hla/alpha.v3.html.
Test systems were developed and their performance characteristics determined by the H&I laboratory at the University of Utah Health, under the accreditation guidelines from the American Society for Histocompatibility and Immunogenetics (ASHI). Some tests or reagents have not been cleared or approved by the FDA.
Order this test for single antigen HLA-DQ identification.
|Component Test Code*||Component Chart Name||LOINC|
|2014080||HLA Class II, Locus DQA1*, Allele 1||59019-0|
|2014081||HLA Class II, Locus DQA1*, Allele 2||59019-0|
|2014082||HLA Class II, Locus DQB1*, Allele 1||57299-0|
|2014083||HLA Class II, Locus DQB1*, Allele 2||57299-0|
|2014084||HLA-DQ Genotyping Interpretation||50398-7|