Ordering Recommendation

May be useful in immunization/vaccination trials or may aid in the clinical diagnosis of diseases strongly associated with the HLA-DP locus.

Mnemonic
HLA-DP DNA
Methodology

Polymerase Chain Reaction/Sequence-Specific Oligonucleotide Probe Hybridization

Performed

Mon-Fri

Reported

3-7 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B).

Specimen Preparation

Transport 5 mL whole blood. (Min: 3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Green (Sodium or Lithium Heparin) tubes.

Remarks
Stability

Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Background Information for HLA-DP Genotyping:
Purpose:
For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-DP locus.
Methodology:
PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-DP locus.
Analytical Sensitivity & Specificity:
Medium to high resolution of HLA-DP locus.
Limitations:
The presence of a disease-associated HLA combination does not establish a diagnosis. If fewer than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation; for pre-transplant allele matching, consider HLA Class I and II Panel (HLA A, HLA B, HLA C, DRB1, DQA1, DQB1, DPB1) by Next Generation Sequencing (ARUP test code 3002061) or HLA Class I and II Panel (HLA A, HLA B, HLA C, DRB1, DRB345, DQA1, DQB1, DPA1, DPB1) by Next Generation Sequencing (ARUP test code 3002062).
Occasionally the specific allele cannot be determined; in this case, the most likely allele assignment is made followed by a sequence of letters indicating other possible allele assignments. Interpretation of allele codes can be found at https://bioinformatics.bethematchclinical.org/hla/alpha.v3.html.

Test systems were developed and their performance characteristics determined by the H&I laboratory at the University of Utah Health, under the accreditation guidelines from the American Society for Histocompatibility and Immunogenetics (ASHI). Some tests or reagents have not been cleared or approved by the FDA.

Compliance Category

Not Applicable

Note

Order this test for single antigen HLA-DP identification.

Hotline History
N/A
CPT Codes

81382

Components
Component Test Code* Component Chart Name LOINC
2014074 HLA Class II, Locus DPA1*, Allele 1 59018-2
2014075 HLA Class II, Locus DPA1*, Allele 2 59018-2
2014076 HLA Class II, Locus DPB1*, Allele 1 59017-4
2014077 HLA Class II, Locus DPB1*, Allele 2 59017-4
2014078 HLA-DP Genotyping Interpretation 50398-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • None
HLA-DP Genotyping