Ordering Recommendation

Testing should only be performed on individuals meeting Centers for Disease Control and Prevention (CDC) clinical criteria for Zika virus (eg, clinical signs and symptoms associated with Zika virus infection) and/or CDC epidemiological criteria for Zika virus (eg, history of residence in or travel to a geographic region with active Zika transmission at the time of travel, or other epidemiological criteria for which Zika virus testing may be indicated).

Mnemonic
ZIKAPCR U
Methodology

Qualitative Polymerase Chain Reaction

Performed

Mon, Wed, Fri

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Urine and patient-matched Serum Separator Tube (SST).

Specimen Preparation

Urine: Transfer 1 mL urine to a sterile container. (Min: 0.5 mL)
Serum: Collect and retain 2 mL of patient-matched serum at the client site in the event that serological follow-up testing is needed. (Min: 1 mL)

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

Serum (refer to Zika Virus by PCR, Blood, ARUP test code 2014065).

Remarks

Specimen source required.

Stability

Ambient: Unacceptable; Refrigerated: 5 days; Frozen: 6 weeks

Reference Interval
Interpretive Data

Zika Virus by PCR is a real-time RT-PCR test intended for the qualitative detection of Zika virus RNA from individuals meeting CDC Zika virus clinical criteria (e.g., clinical signs and symptoms associated with Zika virus infection) and/or CDC Zika virus epidemiological criteria (e.g., history of residence in or travel to a geographic region with active Zika transmission at the time of travel, or other epidemiologic criteria for which Zika virus testing may be indicated).

Health care providers are strongly encouraged to collect serum specimens alongside other specimen types to provide additional opportunities for diagnosing Zika. A positive RT-PCR result confirms Zika virus infection, and no additional testing is indicated. When test results are negative for urine, the patient-matched serum should be tested as outlined in the current CDC-issued algorithm (https://www.cdc.gov/zika/laboratories/lab-guidance.html).

If serologic testing is needed on a patient-matched serum specimen, contact ARUP client services to order Zika Virus IgM Antibody Capture (MAC), by ELISA (ARUP test code 2013942). Additional charges apply.

The Zika Virus by PCR test is for in vitro diagnostic use under the FDA Emergency Use Authorization (EUA). This test has not been FDA cleared or approved. In compliance with this authorization, please visit https://aruplab.com/zika for more information and to access the applicable information sheets.

Compliance Category

Emergency Use Authorization (EUA)

Note
Hotline History
N/A
CPT Codes

87662

Components
Component Test Code* Component Chart Name LOINC
2014070 Zika Virus, Urine, Source 31208-2
2014071 Zika Virus by PCR, Urine 79190-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
Zika Virus by PCR, Urine