Use for patients whose symptoms began, or whose documented exposure occurred, ≥14 days prior to testing. Use as follow-up for patients with negative serum and urine results from molecular testing performed <14 days after symptom onset.
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Mon, Wed, Fri
Serum Separator Tube (SST).
Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1.0 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute or convalescent."
Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.
Submit patient history.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
The possibility of false-positive or false-negative results must be considered. RT-PCR testing on both a serum and urine specimen is recommended by the Centers for Disease Control and Prevention (CDC) to rule out false-negative IgM results in patients experiencing symptoms for less than 2 weeks. Specimens collected for IgM testing greater than or equal to 2 weeks after symptom onset do not require any additional testing. For more information, please review the current clinical guidelines for Zika virus testing at: www.cdc.gov/zika/.
If the result is "Presumptive Zika," then Zika IgM Ab Capture (MAC) Confirmation (ARUP test code 3001904) will be added at no additional charge.
|Component Test Code*||Component Chart Name||LOINC|
|2014017||Zika Virus IgM Ab Capture (MAC), ELISA||80824-6|
|2014019||Relevant Zika virus Exposure?|
|2014020||Symptoms consistent with Zika virus?||75325-1|