Ordering Recommendation

Use for patients whose symptoms began, or whose documented exposure occurred, ≥14 days prior to testing. Use as follow-up for patients with negative serum and urine results from molecular testing performed <14 days after symptom onset.


Semi-Quantitative Enzyme-Linked Immunosorbent Assay


Mon, Wed, Fri


1-6 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1.0 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute or convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.


Submit patient history.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval


Interpretive Data

The possibility of false-positive or false-negative results must be considered. RT-PCR testing on both a serum and urine specimen is recommended by the Centers for Disease Control and Prevention (CDC) to rule out false-negative IgM results in patients experiencing symptoms for less than 2 weeks. Specimens collected for IgM testing greater than or equal to 2 weeks after symptom onset do not require any additional testing. For more information, please review the current clinical guidelines for Zika virus testing at: www.cdc.gov/zika/.

Compliance Category



Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
2014017 Zika Virus IgM Ab Capture (MAC), ELISA 80824-6
2014018 Pregnant? 66174-4
2014019 Relevant Zika virus Exposure? 92944-8
2014020 Symptoms consistent with Zika virus? 75325-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Zika Virus IgM Antibody Capture (MAC), by ELISA