Ordering Recommendation

Use for patients whose symptoms began, or whose documented exposure occurred, at least 14 days prior to testing. Should also be used as follow-up for patients with negative serum and urine results from molecular testing performed less than 14 days after symptom onset.

Mnemonic
ZIKA M
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Mon, Wed, Fri

Reported

1-6 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1.0 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute or convalescent."

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Remarks

Submit patient history.

Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Negative

Interpretive Data

The possibility of false-positive or false-negative results must be considered. RT-PCR testing on both a serum and urine specimen is recommended by the Centers for Disease Control and Prevention (CDC) to rule out false-negative IgM results in patients experiencing symptoms for less than 2 weeks. Specimens collected for IgM testing greater than or equal to 2 weeks after symptom onset do not require any additional testing. For more information, please review the current clinical guidelines for Zika virus testing at: www.cdc.gov/zika/.

Compliance Category

FDA

Note

If the result is "Presumptive Zika," then Zika IgM Ab Capture (MAC) Confirmation (ARUP test code 3001904) will be added at no additional charge.

Hotline History
N/A
CPT Codes

86794

Components
Component Test Code* Component Chart Name LOINC
2014017 Zika Virus IgM Ab Capture (MAC), ELISA 80824-6
2014018 Pregnant? 66174-4
2014019 Relevant Zika virus Exposure?
2014020 Symptoms consistent with Zika virus? 75325-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
Zika Virus IgM Antibody Capture (MAC), by ELISA