Ordering Recommendation

Use for patients whose symptoms began, or whose documented exposure occurred, ≥14 days prior to testing. Use as follow-up for patients with negative serum and urine results from molecular testing performed <14 days after symptom onset.




Semi-Quantitative Enzyme-Linked Immunosorbent Assay


Mon, Wed, Fri


1-6 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1.0 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimen plainly as "acute or convalescent."

Storage/Transport Temperature


Unacceptable Conditions

Bacterially contaminated, heat-inactivated, hemolyzed, icteric, lipemic, or turbid specimens.


Submit patient history.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval


Interpretive Data

The possibility of false-positive or false-negative results must be considered. RT-PCR testing on both a serum and urine specimen is recommended by the Centers for Disease Control and Prevention (CDC) to rule out false-negative IgM results in patients experiencing symptoms for less than 2 weeks. Specimens collected for IgM testing greater than or equal to 2 weeks after symptom onset do not require any additional testing. For more information, please review the current clinical guidelines for Zika virus testing at: www.cdc.gov/zika/.

Compliance Category



If the result is "Presumptive Zika," then Zika IgM Ab Capture (MAC) Confirmation (ARUP test code 3001904) will be added at no additional charge.

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
2014017 Zika Virus IgM Ab Capture (MAC), ELISA 80824-6
2014018 Pregnant? 66174-4
2014019 Relevant Zika virus Exposure? 92944-8
2014020 Symptoms consistent with Zika virus? 75325-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Zika Virus IgM Antibody Capture (MAC), by ELISA