BRAF V600E Mutation Detection in Circulating Cell-Free DNA by Digital Droplet PCR
Determine BRAF V600E mutation status in patients with solid tumors to select candidates for targeted therapy with kinase inhibitors (BRAF and/or MEK). Monitor response to therapy and disease progression in patients carrying BRAF V600E mutation.
Polymerase Chain Reaction
Whole blood in two 10mL Cell-Free DNA (cfDNA) BCT Tubes. Specimens must be collected using the Kit, Cell-Free DNA Blood Collection Tube (ARUP Supply #52358) available online through eSupply using ARUP Connect or contact ARUP Client Services at (800) 522-2787.
Transport 20 mL whole blood in cfDNA BCT Tubes. (Min: 16 mL)
FFPE tissue. Whole blood collected in non-cfDNA BCT tubes.
Ambient: 5 days; Refrigerated: 5 days; Frozen: Unacceptable
Refer to report.
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|2013922||BRAF V600E cfDNA Result||58415-1|
|2013924||BRAF V600E cfDNA Mutant Allele Frequency|
|2013925||BRAF V600E cfDNA Mutant copies/mL|