Natural Killer Cell and Natural Killer T-Cell Panel
Ordering Recommendation
Use to enumerate NK- and NKT-cell populations. This is not a NK-cell activity assay and should not be used as part of the diagnostic criteria for hemophagocytic lymphohistiocytosis (HLH).
New York DOH Approval Status
Specimen Required
Lavender (EDTA), or Pink (K2EDTA).
Transport 4 mL whole blood. (Min: 1 mL)
CRITICAL ROOM TEMPERATURE
Clotted or hemolyzed specimens
Specimens must be analyzed within 48 hours of collection.
New York State Clients: Same as the above.
Room temperature: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Same as the above.
Methodology
Semi-Quantitative Flow Cytometry
Performed
Sun-Sat
Reported
1-3 days
Reference Interval
Test Number |
Components |
Reference Interval |
---|---|---|
Pct CD3-CD16-/+CD56br/dim(total NKcells) | 5.0 - 32.5 % | |
Abs CD3-CD16-/+CD56br/dim(total NKcells) | 91 - 593 cells/uL | |
Pct CD3-CD16+CD56dim(cytotoxic NK-cells) | 47.0 - 94.5 % | |
Abs CD3-CD16+CD56dim(cytotoxic NK-cells) | 51 - 482 cells/uL | |
Pct CD3-CD16-CD56br (cyto secreting NK) | 0.7 - 6.3 % | |
Abs CD3-CD16-CD56br (cyto secreting NK) | 2 - 16 cells/uL | |
Pct CD3+CD56+ (CD56 NKT-cells) | 0.9 - 14.4 % | |
Abs CD3+CD56+ (CD56 NKT-cells) | 20-349 cells/uL | |
Pct CD45+CD3+ (T-cells) | 59.0 - 86.9 % | |
Abs CD45+CD3+ (T-cells) | 654 - 2634 cells/uL | |
Pct CD45+CD3- (Non T-cells) | 15.7 - 43.6 % | |
Abs CD45+CD3- (Non T-cells) | 196 - 897 cells/uL | |
Natural Killer T-cell Panel Interp | See Note |
Interpretive Data
Natural killer (NK) cells are identified by the absence of CD3 and the expression of CD16 and/or CD56. NK cells are divided according to the expression of CD16 and CD56 into cytotoxic NK cells (CD3-CD16+CD56dim) that represents approximately 90 percent of NK cells, and cytokine-secreting or regulatory NK cells (CD3-CD16-CD56bright) that represents approximately 10 percent of NK cells. NK cells act against virally-infected cells and tumor cells and may be increased or decreased in various immunologic abnormalities. NK cells also have a role in the adaptive immune response through cytokine production. NK-like T-cells have properties of both T-cells and NK-cells, expressing both CD3 and NK-associated antigens.
The cytotoxic NK cells (CD3-CD16+CD56dim) and cytokine-secreting or regulatory NK cells (CD3-CD16-CD56bright) are reported as a percentage of the total NK cells. All other populations are reported as a percentage of the total lymphocytes.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Note
Hotline History
CPT Codes
86356 x3; 86357; 86359
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2013806 | Pct CD3-CD16-/+CD56br/dim(total NKcells) | 8112-5 |
2013807 | Abs CD3-CD16-/+CD56br/dim(total NKcells) | 9728-7 |
2013808 | Pct CD3-CD16+CD56dim(cytotoxic NK-cells) | 8112-5 |
2013809 | Abs CD3-CD16+CD56dim(cytotoxic NK-cells) | 9728-7 |
2013810 | Pct CD3-CD16-CD56br (cyto secreting NK) | |
2013811 | Abs CD3-CD16-CD56br (cyto secreting NK) | |
2013814 | Pct CD3+CD56+ (CD56 NKT-cells) | 17135-5 |
2013815 | Abs CD3+CD56+ (CD56 NKT-cells) | 26858-1 |
2013818 | Pct CD45+CD3+ (T-cells) | 56907-9 |
2013819 | Abs CD45+CD3+ (T-cells) | 89312-3 |
2013820 | Pct CD45+CD3- (Non T-cells) | 89311-5 |
2013821 | Abs CD45+CD3- (Non T-cells) | 89313-1 |
2013822 | Natural Killer T-cell Panel Interp | 80722-2 |