Ordering Recommendation

Use only when patient has a repeatedly reactive third- or fourth-generation HIV screen test result. This test discriminates between HIV-1 and HIV-2 antibodies.

Mnemonic
HIV AB SUP
Methodology

Qualitative Immunoassay

Performed

Varies

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA), or Pink (K2 EDTA). Also acceptable: Serum Separator Tube (SST).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma into an ARUP Standard Transport Tube dedicated only for HIV testing. (Min: 0.5 mL) Remove particulate material.

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
HIV-1 Antibody Negative
HIV-2 Antibody Negative

Interpretive Data

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cells, Tissues, and Cellular and Tissue-Based Products (HCT/P).

No compliance statements are in use for this test.

Note

For use ONLY when patient has a repeatedly reactive third- or fourth-generation HIV screen test result. This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported. This test is for use as the antibody differentiation test in the specific multi-test algorithm. If results are negative or indeterminate, this test does NOT reflex to a nucleic acid test.

A multi-test algorithm is recommended by the Centers for Disease Control and Prevention (CDC) and the Clinical Laboratory Standards Institute (CLSI) for the diagnosis of HIV (refer to http://www.arupconsult.com/Topics/HIV.html).

Hotline History
N/A
Components
Component Test Code* Component Chart Name LOINC
2012670 HIV-1/2 Ab Differentiation Immunoassay 8251-1
2012671 HIV-1 Antibody 68961-2
2012672 HIV-2 Antibody 81641-3
2012673 HIV Serologic Interpretation 80203-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Geenius
  • HIV Diagnosis
  • HIV Multispot
  • HIV-1 & 2 Antibody Differentiation
  • HIV-1 and HIV-2 Antibody Differentiation
  • HIV-1 Antibody by Multispot
  • Human Immunodeficiency Virus 1/2 (HIV-1/2) Antibody, Differentiation
Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental