Ordering Recommendation

Screen, diagnose, and monitor diseases associated with excess or deficient cortisol production.


Equilibrium Dialysis/Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry


Sun, Tue, Thu, Fri


2-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Recommended collection times are 8-10 a.m. or 4-6 p.m. 


Plain red or serum separator tube, lavender (EDTA) or pink (K2EDTA).

Specimen Preparation

Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature


Unacceptable Conditions

Grossly hemolyzed, icteric, lipemic or heparinized specimens. 


Indicate time of draw on test request form and specimen tube.


After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 6 months

Reference Interval
Age Time of Collection Reference Range
0 - 17 years Not established
18 years of age and older 8-10 a.m. collection 0.21 - 1.04 µg/dL
18 years of age and older 4-6 p.m. collection 0.10 - 0.63 µg/dL

Interpretive Data

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.


To convert to nmol/L, multiply µg/dL by 27.6.

Hotline History
CPT Codes


Component Test Code* Component Chart Name LOINC
2012698 Cortisol, Free by ED/LC-MS/MS 2145-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Hydrocortisone, Free
Cortisol, Free by Equilibrium Dialysis/LC-MS/MS