Ordering Recommendation

Use as a sensitive alternative to traditional stool culture to detect the most common gastrointestinal bacterial pathogens: Salmonella, Shigella/Enteroinvasive E. coli, Campylobacter jejuni/coli, and shiga-toxigenic E. coli. This test can also detect Campylobacter upsaliensis, which cannot be readily cultured.

Mnemonic
GI BACTPCR
Methodology

Qualitative Polymerase Chain Reaction

Performed

Tue, Thurs, Sat

Reported

2-5 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Stool.

Specimen Preparation

Transfer 1 mL stool to an unpreserved stool transport vial (ARUP Supply #40910), OR place stool in enteric transport media (Cary-Blair) (ARUP supply #29799) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Formalin-fixed stool.

Remarks

This test is New York DOH approved; however, per NYDOH regulations, testing cannot be performed for New York City clients.

Stability

Unpreserved Stool: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 2 months
Stool in Transport Media: Ambient: 1 hour; Refrigerated: 3 days; Frozen: Unacceptable

Reference Interval
Interpretive Data

A negative result does not rule out the presence of PCR inhibitors in the patient specimen or test-specific nucleic acid in concentrations below the level of detection by this test.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note

This assay detects Shigella spp., Enteroinvasive E. coli, Salmonella spp., Campylobacter jejuni/coli, Campylobacter upsaliensis, Shiga-like toxin 1, and Shiga-like toxin 2.

Hotline History
N/A
CPT Codes

87506

Components
Component Test Code* Component Chart Name LOINC
2012679 Shigella/Enteroinvasive E. coli by PCR 46455-2
2012681 Salmonella species by PCR 49612-5
2012683 Campylobacter jejuni/coli by PCR 61368-7
2012685 Campylobacter upsaliensis by PCR 49614-1
2012687 Shiga-like toxin 1 by PCR 63427-9
2012689 Shiga-like toxin 2 by PCR 63428-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
Gastrointestinal Bacterial Panel by PCR