Human Immunodeficiency Virus Types 1 and 2 (HIV-1/2) Antibody Differentiation, Supplemental, with Reflex to HIV-1 Quantitative NAAT, Plasma
Ordering Recommendation
Useful as a supplemental assay for repeatedly reactive third- or fourth-generation HIV screen tests when following the CDC's recommended HIV testing algorithm. This test should not be used as a rapid screen or as a follow-up test for a positive rapid screen.
Methodology
Qualitative Immunoassay/Quantitative Transcription-Mediated Amplification (TMA)
Performed
Varies
Reported
1-2 days
New York DOH Approval Status
Specimen Required
Lavender (EDTA), or pink (K2EDTA).
Separate from cells within 24 hours of collection. Transfer 3 mL plasma into an ARUP standard transport tube dedicated only for HIV testing. (Min: 1 mL) Remove particulate material.
Frozen.
Serum. Heparinized or citrated plasma specimens. Specimens submitted in plasma preparation tube. Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens.
After separation from cells: Ambient: 24 hours (CRITICAL: SHIP FROZEN); Refrigerated:72 hours; Frozen: 3 months (avoid repeated freeze/thaw cycles)
Reference Interval
Effective November 12, 2018
Test Number |
Components |
Reference Interval |
---|---|---|
HIV-1 Antibody | Negative | |
HIV-2 Antibody | Negative | |
3000867 | Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT, Plasma | Not detected |
Interpretive Data
This test should not be used for blood donor screening, associated reentry protocols, or for screening human cells, tissues, and cellular- and tissue-based products (HCT/P).
FDA
Note
For use only when patient has a repeatedly reactive third- or fourth-generation HIV screen test result. This test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported. This test is for use as the antibody differentiation test in the specific multitest algorithm. It is not to be ordered as a rapid screen test and cannot be used as a supplemental test if the initial screen test was a rapid test.
If the HIV-1/ 2 antibody differentiation immunoassay is Negative or Indeterminate, then the Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT, Plasma, will be added. Additional charges apply. Refer to Human Immunodeficiency Virus 1 (HIV-1) by Quantitative NAAT, Plasma (ARUP test code 3000867) for additional information regarding Performed or Reported times, Interpretive Data, and Notes for the reflex test.
The multitest algorithm is recommended by the Centers for Disease Control and Prevention (CDC) and the Clinical Laboratory Standards Institute (CLSI) for the diagnosis of HIV (refer to https://arupconsult.com/content/human-immunodeficiency-virus).
Hotline History
CPT Codes
86701; 86702; if reflexed, add 87536
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2012670 | HIV-1/2 Ab Differentiation Immunoassay | 77202-0 |
2012671 | HIV-1 Antibody | 68961-2 |
2012672 | HIV-2 Antibody | 81641-3 |
2012673 | HIV Serologic Interpretation | 80203-3 |
Aliases
- Geenius
- HIV Diagnosis
- HIV Multispot
- HIV-1 & 2 Antibody Differentiation
- HIV-1 and HIV-2 Antibody Differentiation
- HIV-1 Antibody by Multispot
- Human Immunodeficiency Virus 1/2 (HIV-1/2) Antibody, Differentiation With Reflex to HIV-1 RNA