JAK2 Gene, V617F Mutation, Qualitative with Reflex to CALR (Calreticulin) Exon 9 Mutation Analysis by PCR with Reflex to MPL Mutation Detection
Use when diagnosis of essential thrombocythemia (ET) or primary myelofibrosis (PMF) is suspected.
Polymerase Chain Reaction/Capillary Electrophoresis/Capillary Electrophoresis
DNA Isolation: Sun-Sat
Assay: Mon, Wed, Fri
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA certified lab.
Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely
Refer to report.
Laboratory Developed Test (LDT)
If JAK2 V617F is reported as "Not Detected" then CALR Exon 9 Mutation Analysis by PCR will be added. If CALR is reported as "Not Detected," then MPL Mutation Detection will be added. Additional charges apply.
81270; if reflexed add 81219; if reflexed again add 81338
|Component Test Code*||Component Chart Name||LOINC|
|0051245||JAK2 Gene, V617F Mutation, Qualitative||43399-5|