Ordering Recommendation

Use when diagnosis of essential thrombocythemia (ET) or primary myelofibrosis (PMF) is suspected.




Polymerase Chain Reaction/Capillary Electrophoresis/Capillary Electrophoresis


DNA Isolation: Sun-Sat
Assay: Mon, Wed, Fri


3-6 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Lavender (EDTA) or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA certified lab.

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature


Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.


Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely

Reference Interval

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


If JAK2 V617F is reported as "Not Detected" then CALR Exon 9 Mutation Analysis by PCR will be added. If CALR is reported as "Not Detected," then MPL Mutation Detection will be added. Additional charges apply.

Hotline History


CPT Codes

81270; if reflexed add 81219; if reflexed again add 81338


Component Test Code* Component Chart Name LOINC
0051245 JAK2 Gene, V617F Mutation, Qualitative 43399-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


JAK2 Gene, V617F Mutation, Qualitative with Reflex to CALR (Calreticulin) Exon 9 Mutation Analysis by PCR with Reflex to MPL Mutation Detection (INACTIVE as of 08/16/21: Refer to 3003800 in the August Hotline)