SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
Ordering Recommendation
May be useful as a secondary screen based on results of ANA test or if ANA IFA is negative and Sjögren syndrome, systemic lupus erythematosus (SLE), systemic sclerosis, or myositis is strongly suspected.
New York DOH Approval Status
Specimen Required
Serum separator tube (SST).
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)
Refrigerated.
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-3 days
Reference Interval
Test Number |
Components |
Reference Interval |
---|---|---|
SSA-52 (Ro52) (ENA) Antibody, IgG | 40 AU/mL or less | |
SSA-60 (Ro60) (ENA) Antibody, IgG | 40 AU/mL or less |
Interpretive Data
SSA-52 (Ro52) and/or SSA-60 (Ro60) antibodies are associated with a diagnosis of Sjögren syndrome, systemic lupus erythematosus (SLE), and systemic sclerosis. SSA-52 antibody overlaps significantly with the major SSc-related antibodies. SSA-52 (Ro52) antibody occurs frequently in patients with inflammatory myopathies, often in the presence of interstitial lung disease.
Component | Interpretation |
---|---|
SSA-52 (Ro52) (ENA) Antibody, IgG | 29 AU/mL or Less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive |
SSA-60 (Ro60) (ENA) Antibody, IgG | 29 AU/mL or Less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive |
FDA
Note
Hotline History
Hotline History
CPT Codes
86235 x2
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0050691 | SSA-52 (Ro52) (ENA) Antibody, IgG | 31625-7 |
2012055 | SSA-60 (Ro60) (ENA) Antibody, IgG | 53019-6 |
Aliases
- Anti-Ro
- Anti-SSA; Anti-SSA (Ro) (ENA) Antibody
- Ro
- Ro Autoantibodies
- Sjogren Syndrome
- Sjogren's Antibody
- SSA Autoantibodies