SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
Ordering Recommendation
May be useful as a secondary screen based on results of ANA test or if ANA IFA is negative and Sjögren syndrome, systemic lupus erythematosus (SLE), systemic sclerosis, or myositis is strongly suspected.
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
Specimen Required
Serum separator tube (SST).
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)
Refrigerated.
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval
Test Number |
Components |
Reference Interval |
---|---|---|
SSA-52 (Ro52) (ENA) Antibody, IgG | 40 AU/mL or less | |
SSA-60 (Ro60) (ENA) Antibody, IgG | 40 AU/mL or less |
Interpretive Data
SSA-52 (Ro52) and/or SSA-60 (Ro60) antibodies are associated with a diagnosis of Sjögren syndrome, systemic lupus erythematosus (SLE), and systemic sclerosis. SSA-52 antibody overlaps significantly with the major SSc-related antibodies. SSA-52 (Ro52) antibody occurs frequently in patients with inflammatory myopathies, often in the presence of interstitial lung disease.
Component | Interpretation |
---|---|
SSA-52 (Ro52) (ENA) Antibody, IgG | 29 AU/mL or Less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive |
SSA-60 (Ro60) (ENA) Antibody, IgG | 29 AU/mL or Less: Negative 30-40 AU/mL: Equivocal 41 AU/mL or greater: Positive |
FDA
Note
Hotline History
Hotline History
CPT Codes
86235 x2
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0050691 | SSA-52 (Ro52) (ENA) Antibody, IgG | 31625-7 |
2012055 | SSA-60 (Ro60) (ENA) Antibody, IgG | 53019-6 |
Aliases
- Anti-Ro
- Anti-SSA; Anti-SSA (Ro) (ENA) Antibody
- Ro
- Ro Autoantibodies
- Sjogren Syndrome
- Sjogren's Antibody
- SSA Autoantibodies