Ordering Recommendation

May be useful as a secondary screen based on results of ANA test or if ANA IFA is negative and Sjögren syndrome, systemic lupus erythematosus (SLE), systemic sclerosis, or myositis is strongly suspected.

Mnemonic
SSA RO
Methodology

Semi-Quantitative Multiplex Bead Assay

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube (SST).

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
SSA-52 (Ro52) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG 29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Interpretive Data

SSA-52 (Ro52) and/or SSA-60 (Ro60) antibodies are associated with a diagnosis of Sjögren syndrome, systemic lupus erythematosus (SLE), and systemic sclerosis. SSA-52 antibody overlaps significantly with the major SSc-related antibodies. SSA-52 (Ro52) antibody occurs frequently in patients with inflammatory myopathies, often in the presence of interstitial lung disease.

No compliance statements are in use for this test.

Note
Hotline History
N/A
CPT Codes

86235 x2

Components
Component Test Code* Component Chart Name LOINC
0050691 SSA-52 (Ro52) (ENA) Antibody, IgG 31625-7
2012055 SSA-60 (Ro60) (ENA) Antibody, IgG 53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Anti-Ro
  • Anti-SSA; Anti-SSA (Ro) (ENA) Antibody
  • Ro
  • Ro Autoantibodies
  • Sjogren Syndrome
  • Sjogren's Antibody
  • SSA Autoantibodies
SSA 52 and 60 (Ro) (ENA) Antibodies, IgG