May be used to diagnose chikungunya viral infection during acute phase of disease (>5 days after symptom onset). Testing should also be considered for other arthropod-borne viruses with similar symptomology based on clinical presentation and travel history.
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Serum separator tube.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute or convalescent."
Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
|0.79 Index or less||Negative: No significant level of Chikungunya IgM antibody detected.|
|0.80-1.09 Index||Equivocal: Questionable presence of Chikungunya IgM antibody detected. Repeat testing in 10-14 days may be helpful.|
|1.10 Index or greater||Positive: Chikungunya IgM antibody detected.|
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|2011811||Chikungunya Antibody, IgM||83067-9|