Ordering Recommendation

Optimize drug therapy and monitor patient adherence.

Mnemonic
PENTOBAR
Methodology

Quantitative Gas Chromatography/Mass Spectrometry

Performed

Sat

Reported

1-8 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum Pre-dose (Trough) Draw - At a Steady State Concentration or Plasma Pre-dose (Trough) Draw - At a Steady State Concentration in Plain Red, Lavender (K2EDTA), Lavender (K3EDTA), or Pink (K2EDTA).

Specimen Preparation

Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Whole blood. Gel Separator Tubes, Light Blue (Sodium Citrate), or Yellow (SPS or ACD Solution).

Remarks
Stability

Ambient: 3 months; Refrigerated: 3 months; Frozen: 1 year

Reference Interval
Therapeutic Range Sedation: 1-5 µg/mL
Intracranial Pressure (ICP) Therapy: 25-35 µg/mL
Coma: 10-50 µg/mL
Toxic Level Greater than 10 µg/mL

Interpretive Data

The therapeutic range is based on serum pre-dose (trough) draw at steady-state concentration. Toxic concentrations may cause respiratory depression, hypotension, coma and death.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

80345 (Alt code: G0480)

Components
Component Test Code* Component Chart Name LOINC
2011554 Pentobarbital, Ser/Pla 3924-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Nembutal
  • pentobarbital blood level
  • Pentobarbitone
Pentobarbital, Serum or Plasma