Ordering Recommendation

Detects ALK fusion proteins (IHC) and ALK gene rearrangements (FISH) in solid tumors.

Mnemonic
ALK REFLEX
Methodology

Immunohistochemistry/Fluorescence in situ Hybridization

Performed

Mon-Fri

Reported

1-5 days; 12 days if reflexed

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Tumor tissue.

Specimen Preparation

Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 7 unstained (4-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808 recommended but not required) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min. 4 slides). If sending precut slides, do not oven bake.

Storage/Transport Temperature

Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.

Unacceptable Conditions

Paraffin block with no tumor tissue remaining; specimens fixed in any fixative other than 10 percent neutral buffered formalin.

Remarks

Include surgical pathology report.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable.

Reference Interval
Interpretive Data

Refer to report

No compliance statements are in use for this test.

Note

Note: This test includes pathologist interpretation of results. Include surgical pathology report with specimen submitted for testing. Indicate tissue site on requisition. ALK (D5F3) by Immunohistochemistry will reflex to FISH if result is Equivocal or Positive. Additional charges apply.

Hotline History
N/A
CPT Codes

88342; if reflexed, add 88366

Components
Component Test Code* Component Chart Name LOINC
2007325 ALK(D5F3) by IHC Reference Number 57723-9
2007327 ALK(D5F3) by IHC Result 35474-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ALK D5F3 antibody staining
  • ALK D5F3 staining
  • ALK rearrangement by IHC
  • ALK rearrangement staining
ALK (D5F3) by Immunohistochemistry with Reflex to ALK Gene Rearrangements by FISH