Test is not intended for HLA-related disease screening or diagnosis (eg, celiac disease, narcolepsy, rheumatologic diseases).
Polymerase Chain Reaction/Massively Parallel Sequencing
Lavender (EDTA). Also acceptable: Yellow (ACD Solution A).
Transfer 4 mL whole blood to an ARUP Standard Transport Tube. (Min: 1 mL).
Yellow (ACD Soultion B) specimens. Clotted, grossly hemolyzed, or heparinized specimens.
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Purpose: To identify HLA-DRB1 and DQB1 allelic polymorphisms on specimens for transplant candidates and their donors.
Methodology: PCR followed by next generation sequencing of HLA-DRB1 and DQB1 loci.
Analytical Sensitivity & Specificity: >99 percent.
Limitations: Rare diagnostic errors can occur due to primer site mutations.
Test Results: Results are reported as HLA locus (DRB1, or DQB1)* followed by the four digit assigned allele.
No compliance statements are in use for this test.
|Component Test Code*||Component Chart Name||LOINC|
|2011273||HLA Class II - Locus DQB1*, Allele 1|
|2011274||HLA Class II - Locus DQB1*, Allele 2|
|2011275||HLA Class II - Locus DRB1*, Allele 1|
|2011276||HLA Class II - Locus DRB1*, Allele 2|
|2011277||HLA Class II Sequencing, Interpretation|
- Human leukocyte antigen
Children’s Hospital of Philadelphia