Ordering Recommendation

Test is not intended for HLA-related disease screening or diagnosis (eg, celiac disease, narcolepsy, rheumatologic diseases).

Mnemonic
HLA II NGS
Methodology

Polymerase Chain Reaction/Massively Parallel Sequencing

Performed

Varies

Reported

8-15 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA).  Also acceptable: Yellow (ACD Solution A).

Specimen Preparation

Transfer 4 mL whole blood to an ARUP Standard Transport Tube. (Min: 1 mL).

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Yellow (ACD Soultion B) specimens.  Clotted, grossly hemolyzed, or heparinized specimens.

Remarks
Stability

Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Purpose: To identify HLA-DRB1 and DQB1 allelic polymorphisms on specimens for transplant candidates and their donors.
Methodology: PCR followed by next generation sequencing of HLA-DRB1 and DQB1 loci.
Analytical Sensitivity & Specificity: >99 percent.
Limitations: Rare diagnostic errors can occur due to primer site mutations.
Test Results: Results are reported as HLA locus (DRB1, or DQB1)* followed by the four digit assigned allele.

No compliance statements are in use for this test.

Note
Hotline History
N/A
Components
Component Test Code* Component Chart Name LOINC
2011273 HLA Class II - Locus DQB1*, Allele 1
2011274 HLA Class II - Locus DQB1*, Allele 2
2011275 HLA Class II - Locus DRB1*, Allele 1
2011276 HLA Class II - Locus DRB1*, Allele 2
2011277 HLA Class II Sequencing, Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HLA-DQB1
  • HLA-DRB1
  • Human leukocyte antigen
HLA Class II Panel (DRB1 and DQB1) by Next Generation Sequencing

Children’s Hospital of Philadelphia