Test is intended for pre-transplant allele matching. Do not use for specific disease screening or diagnosis (eg, celiac disease, rheumatologic diseases).
Polymerase Chain Reaction/Massively Parallel Sequencing
Lavender (EDTA). Also acceptable Yellow (ACD Solution A)
Transfer 4 mL whole blood to an ARUP Standard Transport Tube. (Min: 1 mL)
Yellow (ACD Solution B) specimens. Clotted, grossly hemolyzed, or heparinized specimens.
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Purpose: To identify HLA-A, -B, and -C allelic polymorphisms on specimens for transplant candidates and their donors.
Methodology: PCR followed by next generation sequencing of HLA-A, -B and -C loci.
Analytical Sensitivity & Specificity: >99 percent.
Limitations: Rare diagnostic errors can occur due to primer site mutations.
Test Results: Results are reported as HLA locus (A, B, or C)* followed by the four digit assigned allele.
No compliance statements are in use for this test.
|Component Test Code*||Component Chart Name||LOINC|
|2011265||HLA Class I - Locus B*, Allele 1||13299-3|
|2011266||HLA Class I - Locus B*, Allele 2||13299-3|
|2011267||HLA Class I - Locus A*, Allele 1||38548-4|
|2011268||HLA Class I - Locus A*, Allele 2||38548-4|
|2011269||HLA Class I - Locus C*, Allele 1||42663-5|
|2011270||HLA Class I - Locus C*, Allele 2||42663-5|
|2011271||HLA-ABC Sequencing, Interpretation||53767-0|
- Human leukocyte antigen
Children’s Hospital of Philadelphia