Ordering Recommendation

Test is intended for pre-transplant allele matching. Do not use for specific disease screening or diagnosis (eg, celiac disease, rheumatologic diseases).

Mnemonic
HLA I NGS
Methodology

Polymerase Chain Reaction/Massively Parallel Sequencing

Performed

Varies

Reported

8-15 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA).  Also acceptable Yellow (ACD Solution A)

Specimen Preparation

Transfer 4 mL whole blood to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Yellow (ACD Solution B) specimens.  Clotted, grossly hemolyzed, or heparinized specimens.

Remarks
Stability

Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Purpose: To identify HLA-A, -B, and -C allelic polymorphisms on specimens for transplant candidates and their donors.
Methodology: PCR followed by next generation sequencing of HLA-A, -B and -C loci.
Analytical Sensitivity & Specificity: >99 percent.
Limitations: Rare diagnostic errors can occur due to primer site mutations.
Test Results: Results are reported as HLA locus (A, B, or C)* followed by the four digit assigned allele.

No compliance statements are in use for this test.

Note
Hotline History
N/A
Components
Component Test Code* Component Chart Name LOINC
2011265 HLA Class I - Locus B*, Allele 1 13299-3
2011266 HLA Class I - Locus B*, Allele 2 13299-3
2011267 HLA Class I - Locus A*, Allele 1 38548-4
2011268 HLA Class I - Locus A*, Allele 2 38548-4
2011269 HLA Class I - Locus C*, Allele 1 42663-5
2011270 HLA Class I - Locus C*, Allele 2 42663-5
2011271 HLA-ABC Sequencing, Interpretation 53767-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HLA-A
  • HLA-B
  • HLA-C
  • Human leukocyte antigen
HLA Class I Panel (ABC) by Next Generation Sequencing

Children’s Hospital of Philadelphia