Ordering Recommendation

Preferred test to detect and genotype herpes simplex virus (HSV) types 1 and 2.




Qualitative Polymerase Chain Reaction




1-4 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Lavender (EDTA), pink (K2EDTA), serum separator tube. OR CSF, bronchoalveolar lavage (BAL), amniotic fluid, vesicle fluid, ocular fluid, tissue. OR endocervical specimen in ThinPrep Pap Test media.

Specimen Preparation

Separate plasma or serum from cells. Transfer 1 mL plasma, serum, CSF, BAL, amniotic fluid, ocular fluid or ThinPrep specimen to a sterile container. (Min: 0.5 mL) 
Transfer to a sterile container and freeze immediately.
Vesicle Fluid: Transfer to viral transport media (ARUP supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature


Unacceptable Conditions

Heparinized specimens, tissues in optimal cutting temperature compound.


Specimen source required.


Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 3 months
All others: Ambient: 8 hours; Refrigerated: 72 hours; Frozen: 3 months

Reference Interval

Available Separately Components
Yes (0060041) Herpes Simplex Virus by PCR
Yes (2010095) Herpes Simplex Virus (HSV-1/HSV-2) Subtype by PCR

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


If Herpes Simplex Virus by PCR result is detected, then Herpes Simplex Virus (HSV-1/HSV-2) Subtype by PCR will be added. Additional charges apply.

Hotline History


CPT Codes

87529; if reflexed, add 87529 x2


Component Test Code* Component Chart Name LOINC
0050102 Herpes Simplex Virus Source 31208-2
0060041 Herpes Simplex Virus by PCR 5014-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Herpes Simplex Virus (HSV) by PCR with Reflex to HSV (HSV-1/HSV-2) Subtype by PCR