Ordering Recommendation
Use to identify eosinophil involvement/eosinophil-related inflammation by staining for the eosinophil granule core protein, major basic protein 1 (eMBP1), and/or the eosinophil granule matrix protein, eosinophil-derived neurotoxin (EDN) in order to detect cellular localization and extracellular deposition.
Eosinophil granule proteins are toxic to tissues and cells and may be pathogenic in disease but may not be recognized when deposited outside of morphologically-identifiable intact eosinophils.
Mnemonic
Methodology
Immunofluorescence/Affinity Chromatography
Performed
Varies
Reported
1-3 weeks
New York DOH Approval Status
Specimen Required
Tissue.
Preferred: Transport tissue (optimal 3-8 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. (Min: 1 mm).
Also acceptable: Zeus tissue fixative; flash frozen fresh tissue; formalin-fixed tissue; or formalin-fixed, paraffin-embedded tissue (see Note Section below). Transport in formalin, tissue block, or slides with two 4-micron sections per slide in serial order and numbered. (Min: 8 slides; sections close together in middle of slide).
Room temperature.
Flash Frozen Fresh Tissue: Frozen
Michel's Medium or Zeus Tissue Fixative: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Formalin Fixed Tissue: Ambient: 3 months; Refrigerated: 3 months; Frozen: Unacceptable
Flash Frozen Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Indefinitely
Paraffin Embedded Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Indefinitely
Reference Interval
By report
Interpretive Data
Performed by non-ARUP Laboratory
Note
Turnaround time may be prolonged on specimens submitted as formalin-fixed, paraffin-embedded (FFPE) tissue; clients should consider contacting the Immunodermatology Laboratory when submitting FFPE tissue for Eosinophil Granule Major Basic Protein, Tissue to discuss specific clinical information that may indicate expedited testing and resulting. Contact ARUP Client Services at 1-800-242-2787, option 2, and ask to speak with the Immunodermatology Laboratory at the University of Utah regarding patient results and/or testing information.
Hotline History
CPT Codes
88305; 88342
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
0097167 | SO Source | 31208-2 |
2010911 | EER Eosinophil Granule MBP in Tissues | 11526-1 |
2010922 | Eosinophil Granule MBP in Tissues | 10478-6 |