Use to recognize eosinophil involvement/eosinophil-related inflammation with indirect immunofluorescence to detect cellular localization and extracellular deposition of the eosinophil granule core protein (major basic protein 1 [eMBP1]), and/or the eosinophil granule matrix protein (eosinophil-derived neurotoxin [EDN]) in tissues or organs with suspected eosinophil-driven pathophysiology.
For more information on specimen collection and diseases assessed, see the ARUP Immunobullous Disease Testing Comparison tool.
Indirect Immunofluorescence (IIF)
New York DOH Approval Status
Transport tissue (optimal 3-8 mm) in Michel medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. (Min: 1 mm).
Also acceptable: Zeus tissue fixative, flash frozen fresh tissue, formalin fixed tissue, or formalin fixed and paraffin embedded tissue. Transport in formalin, tissue block, or slides with two 4-5-micron sections per slide in serial order and numbered. (Min: 8 slides).
Flash Frozen Fresh Tissue: Frozen
Michel Medium or Zeus Tissue Fixative: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Formalin Fixed Tissue: Ambient: 3 weeks; Refrigerated: 3 weeks; Frozen: Unacceptable
Flash Frozen Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Indefinitely
Paraffin Embedded Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Indefinitely
Refer to report
Performed by non-ARUP Laboratory
Turnaround time may be prolonged on specimens submitted as formalin-fixed, paraffin-embedded (FFPE) tissue; clients should consider contacting the Immunodermatology Laboratory when submitting FFPE tissue for Eosinophil Granule Major Basic Protein, Tissue to discuss specific clinical information that may indicate expedited testing and resulting. Contact ARUP Client Services at 1-800-242-2787, option 2, and ask to speak with the Immunodermatology Laboratory at the University of Utah regarding patient results and/or testing information.
|Component Test Code*||Component Chart Name||LOINC|
|2010911||EER Eosinophil Granule MBP in Tissues||11526-1|
|2010922||Eosinophil Granule MBP in Tissues||10478-6|
- Eosinophil Granule Major Basic Protein, Biopsy
- eosinophil MBP
- eosinophil-associated disease (EAD)
- eosinophil-related disease
- eosinophilic inflammation