Ordering Recommendation

Use to identify eosinophil involvement/eosinophil-related inflammation by staining for the eosinophil granule core protein, major basic protein 1 (eMBP1), and/or the eosinophil granule matrix protein, eosinophil-derived neurotoxin (EDN) in order to detect cellular localization and extracellular deposition.

Eosinophil granule proteins are toxic to tissues and cells and may be pathogenic in disease but may not be recognized when deposited outside of morphologically-identifiable intact eosinophils.

Mnemonic

EGMBP TIS

Methodology

Immunofluorescence/Affinity Chromatography

Performed

Varies

Reported

1-3 weeks

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation
Collect

Tissue.

Specimen Preparation

Preferred: Transport tissue (optimal 3-8 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. (Min: 1 mm).
Also acceptable: Zeus tissue fixative; flash frozen fresh tissue; formalin-fixed tissue; or formalin-fixed, paraffin-embedded tissue (see Note Section below). Transport in formalin, tissue block, or slides with two 4-micron sections per slide in serial order and numbered. (Min: 8 slides; sections close together in middle of slide).

Storage/Transport Temperature

Room temperature.
Flash Frozen Fresh Tissue: Frozen

Unacceptable Conditions
Remarks
Stability

Michel's Medium or Zeus Tissue Fixative: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Formalin Fixed Tissue: Ambient: 3 months; Refrigerated: 3 months; Frozen: Unacceptable
Flash Frozen Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Indefinitely
Paraffin Embedded Tissue: Ambient:  Indefinitely; Refrigerated:  Indefinitely; Frozen: Indefinitely

Reference Interval

By report

Interpretive Data



Compliance Category

Performed by non-ARUP Laboratory

Note

Turnaround time may be prolonged on specimens submitted as formalin-fixed, paraffin-embedded (FFPE) tissue; clients should consider contacting the Immunodermatology Laboratory when submitting FFPE tissue for Eosinophil Granule Major Basic Protein, Tissue to discuss specific clinical information that may indicate expedited testing and resulting. Contact ARUP Client Services at 1-800-242-2787, option 2, and ask to speak with the Immunodermatology Laboratory at the University of Utah regarding patient results and/or testing information.

Hotline History

N/A

CPT Codes

88305; 88342

Components

Component Test Code* Component Chart Name LOINC
0097167 SO Source 31208-2
2010911 EER Eosinophil Granule MBP in Tissues 11526-1
2010922 Eosinophil Granule MBP in Tissues 10478-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

Eosinophil Granule Major Basic Protein, Tissue