Use to identify eosinophil involvement/eosinophil-related inflammation by staining for the eosinophil granule core protein, major basic protein 1 (eMBP1), and/or the eosinophil granule matrix protein, eosinophil-derived neurotoxin (EDN) in order to detect cellular localization and extracellular deposition.
Eosinophil granule proteins are toxic to tissues and cells and may be pathogenic in disease but may not be recognized when deposited outside of morphologically-identifiable intact eosinophils.
Transport tissue (optimal 3-8 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. (Min: 1 mm).
Also acceptable: Zeus tissue fixative, flash frozen fresh tissue, formalin fixed tissue, or formalin fixed and paraffin embedded tissue. Transport in formalin, tissue block, or slides with two 5-micron sections per slide in serial order and numbered. (Min: 8 slides).
Flash Frozen Fresh Tissue: Frozen
Michel's Medium or Zues Tissue Fixative: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Formalin Fixed Tissue: Ambient: 3 weeks; Refrigerated: 3 weeks; Frozen: Unacceptable
Flash Frozen Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Indefinitely
Paraffin Embedded Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Indefinitely
|Component Test Code*||Component Chart Name||LOINC|
|2010911||EER Eosinophil Granule MBP in Tissues||11526-1|
|2010922||Eosinophil Granule MBP in Tissues||10478-6|