Ordering Recommendation

Use to recognize eosinophil involvement/eosinophil-related inflammation with indirect immunofluorescence to detect cellular localization and extracellular deposition of the eosinophil granule core protein (major basic protein 1 [eMBP1]), and/or the eosinophil granule matrix protein (eosinophil-derived neurotoxin [EDN]) in tissues or organs with suspected eosinophil-driven pathophysiology. 

For more information on specimen collection and diseases assessed, see the ARUP Immunobullous Disease Testing Comparison tool.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation


Specimen Preparation

Transport tissue (optimal 3-8 mm) in Michel medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. (Min: 1 mm).
Also acceptable: Zeus tissue fixative, flash frozen fresh tissue, formalin fixed tissue, or formalin fixed and paraffin embedded tissue. Transport in formalin, tissue block, or slides with two 4-5-micron sections per slide in serial order and numbered. (Min: 8 slides).

Storage/Transport Temperature

Room temperature.
Flash Frozen Fresh Tissue: Frozen

Unacceptable Conditions

Michel Medium or Zeus Tissue Fixative: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Formalin Fixed Tissue: Ambient: 3 weeks; Refrigerated: 3 weeks; Frozen: Unacceptable
Flash Frozen Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Indefinitely
Paraffin Embedded Tissue: Ambient:  Indefinitely; Refrigerated:  Indefinitely; Frozen: Indefinitely


Indirect Immunofluorescence (IIF)




7-21 days

Reference Interval

By report

Interpretive Data

Refer to report

Compliance Category

Performed by non-ARUP Laboratory


Turnaround time may be prolonged on specimens submitted as formalin-fixed, paraffin-embedded (FFPE) tissue; clients should consider contacting the Immunodermatology Laboratory when submitting FFPE tissue for Eosinophil Granule Major Basic Protein, Tissue to discuss specific clinical information that may indicate expedited testing and resulting. Contact ARUP Client Services at 1-800-242-2787, option 2, and ask to speak with the Immunodermatology Laboratory at the University of Utah regarding patient results and/or testing information.

Hotline History


CPT Codes

88305; 88342


Component Test Code* Component Chart Name LOINC
0097167 SO Source 31208-2
2010911 EER Eosinophil Granule MBP in Tissues 11526-1
2010922 Eosinophil Granule MBP in Tissues 10478-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Eosinophil Granule Major Basic Protein, Biopsy
  • eosinophil MBP
  • eosinophil-associated disease (EAD)
  • eosinophil-related disease
  • eosinophilic
  • eosinophilic inflammation
Eosinophil Granule Major Basic Protein, Tissue Biopsy