Ordering Recommendation

Use to identify eosinophil involvement/eosinophil-related inflammation by staining for the eosinophil granule core protein, major basic protein 1 (eMBP1), and/or the eosinophil granule matrix protein, eosinophil-derived neurotoxin (EDN) in order to detect cellular localization and extracellular deposition.

Eosinophil granule proteins are toxic to tissues and cells and may be pathogenic in disease but may not be recognized when deposited outside of morphologically-identifiable intact eosinophils.

Mnemonic
EGMBP TIS
Methodology

Immunofluorescence/Affinity Chromatography

Performed

Varies

Reported

1-3 weeks

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Tissue.

Specimen Preparation

Transport tissue (optimal 3-8 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. (Min: 1 mm).
Also acceptable: Zeus tissue fixative, flash frozen fresh tissue, formalin fixed tissue, or formalin fixed and paraffin embedded tissue. Transport in formalin, tissue block, or slides with two 5-micron sections per slide in serial order and numbered. (Min: 8 slides).

Storage/Transport Temperature

Room temperature.
Flash Frozen Fresh Tissue: Frozen

Unacceptable Conditions
Remarks
Stability

Michel's Medium or Zues Tissue Fixative: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Formalin Fixed Tissue: Ambient: 3 weeks; Refrigerated: 3 weeks; Frozen: Unacceptable
Flash Frozen Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Indefinitely
Paraffin Embedded Tissue: Ambient:  Indefinitely; Refrigerated:  Indefinitely; Frozen: Indefinitely

Reference Interval

By report

Interpretive Data



Compliance Category

Not Applicable

Note
Hotline History
N/A
CPT Codes

88305; 88342

Components
Component Test Code* Component Chart Name LOINC
0097167 SO Source 31208-2
2010911 EER Eosinophil Granule MBP in Tissues 11526-1
2010922 Eosinophil Granule MBP in Tissues 10478-6
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Aliases
Eosinophil Granule Major Basic Protein, Tissue