Ordering Recommendation

Panel includes PCR testing to detect M. tuberculosis complex isolates and determine possible resistance to rifampin treatment. Test may be ordered for client-processed specimens. Refer to specimen requirements.

Mnemonic
MTBRIF PCR
Methodology

Qualitative Polymerase Chain Reaction

Performed

Sun-Sat

Reported

1-3 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Respiratory specimens, CSF, or Pleural Fluid.

Specimen Preparation

Unprocessed Specimens: Transport 5-10 mL respiratory specimen, CSF or pleural fluid in a sterile container. (Min: 1 mL) Label as unprocessed.
Processed Specimens: Transport 2-5 mL digested/decontaminated respiratory specimen, CSF or pleural fluid in a sterile container. (Min: 1 mL)
Place each specimen in an individually sealed bag.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Blood, paraffin blocks, stool, swabs, tissue, and urine.

Remarks

Specimen source required. Processed Specimens: Identify method used for digestion and provide smear results.

Stability

Unprocessed: Ambient: 3 days; Refrigerated: 1 week; Frozen: 1 month 
Processed
: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 1 month

Reference Interval
Interpretive Data



No compliance statements are in use for this test.

Note

Body Fluids other than Pleural Fluid will be run with a disclaimer.

Specimen source required. To perform this test it is essential to know whether or not the submitted specimen has been processed (digestion and decontamination procedure). If processed, smear results must be provided as a comment on the test order or requisition. Delayed turnaround time will occur if the required information is not provided.

After a positive result, repeat orders for Mycobacterium tuberculosis Complex Detection and Rifampin Resistance by PCR will continue to yield a positive result and repeat testing is not clinically indicated.

Hotline History
N/A
CPT Codes

87556; 87798

Components
Component Test Code* Component Chart Name LOINC
2010776 MTBRIF Source 31208-2
2010777 Mycobacterium Tuberculosis by PCR 13956-8
2010778 MTB Rifampin by PCR 48176-2
2010779 MTB Cmplx Interpretation 48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • MC MTD
  • MC MTDCSF
  • MTB PCR
  • MTD PCR
Mycobacterium tuberculosis Complex Detection and Rifampin Resistance by PCR