MYD88 L265P Mutation Detection by PCR, Quantitative
Ordering Recommendation
Useful in distinguishing lymphoplasmacytic lymphoma (LPL) from other low-grade B-cell lymphoproliferative disorders which may be in the differential diagnosis. Use when monitoring patients with LPL diagnosis and previously identified MYD88 L265P mutation.
New York DOH Approval Status
Specimen Required
Whole blood or bone marrow in lavender (EDTA) or FFPE tumor tissue.
Whole Blood : Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow : Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
FFPE Tumor Tissue : Formalin-fixed (10 percent neutral buffered formalin) and paraffin-embedded tissue. Protect from excessive heat. Transport tissue in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect(TM) or contact ARUP Client Services at 800-522-2787.
Whole Blood, Bone Marrow: Refrigerated
FFPE Tumor Tissue : Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.
Plasma, serum. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens.
FFPE Tumor Tissue: Specimens fixed in any fixative other than 10 percent neutral buffered formalin.
Bone specimens submitted in non-EDTA decalcifier.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.
Whole Blood or Bone Marrow: Refrigerated: 7 days; Frozen: Unacceptable
FFPE Tumor Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Methodology
Real-Time Polymerase Chain Reaction
Performed
Varies
Reported
5-10 days
Reference Interval
Interpretive Data
Refer to report.
Laboratory Developed Test (LDT)
Note
Hotline History
Hotline History
CPT Codes
81305
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2009319 | MYD88 Source | 66746-9 |
2009320 | MYD88 L265P Detection, Result | |
2009321 | MYD88 L265P Detection, Quant | 82140-5 |
Aliases
- Lymphoplasmacytic lymphoma
- Waldenstrom macroglobulinemia