Ordering Recommendation

Aid in treatment decisions in individuals with high-grade gliomas.

Mnemonic
MGMT
Methodology

Polymerase Chain Reaction/MassARRAY/MALDI-TOF

Performed

DNA isolation: Sun-Sat
Assay: Varies

Reported

8-12 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Tumor tissue. Also acceptable: DNA extracted by CLIA certified lab with corresponding client-circled H&E slide.

Specimen Preparation

Tumor Tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Tissue block will be returned after testing. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Transport block and/or slide(s) in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP ConnectTM or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Room temperature. Ship in cooled container during summer months.
Extracted DNA: Refrigerated.

Unacceptable Conditions

Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens. Less than 25 percent tumor.

Remarks

Include surgical pathology report.

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerated: Indefinitely; Frozen: Indefinitely

Reference Interval
Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

88381; 81287

Components
Component Test Code* Component Chart Name LOINC
2002148 Block ID 57723-9
2009311 MGMT Methylation Detection - Results 60252-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
MGMT Promoter Methylation Detection