Ordering Recommendation

HIV-1 genotyping provides antiretroviral susceptibility information for protease inhibitors (PI), reverse transcriptase inhibitors (NRTI, NNRTI), and integrase inhibitors. Intended for patients with viral load >1000 copies/mL.

Mnemonic
HIV1 PRI
Methodology

Polymerase Chain Reaction/Sequencing

Performed

Sun-Sat

Reported

7-10 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA) or plasma preparation tube.

Specimen Preparation

Separate plasma from cells within 6 hours. Transfer 4 mL plasma to an ARUP Standard Transport Tube. (Min: 3 mL)

Storage/Transport Temperature

Frozen.

Unacceptable Conditions

Serum. Heparinized specimens.

Remarks

Please submit most recent viral load and test date, if available.

Stability

On Cells: Ambient: 6 hours; After separation from Cells: Ambient 24 hours; Refrigerated: 5 days; Frozen: 4 months

Reference Interval
Available Components Reference Interval
Yes (0055670) Human Immunodeficiency Virus 1, Genotype by Sequencing Refer to report
Yes (2004457) HIV-1 Integrase Inhibitor Resistance by Sequencing Refer to report

Interpretive Data

Refer to individual components.

Compliance Category

Modified FDA

Note

This test may be unsuccessful if the plasma HIV-1 RNA viral load is less than 1,000 HIV-1 RNA copies per mL of plasma.

Hotline History
N/A
CPT Codes

87901; 87906

Components
Component Test Code* Component Chart Name LOINC
0051722 HIV-1 Genotype by Sequencing 53798-5
2004458 HIV-1 Integrase Inhib. Resistance, Seq 72560-6
2010245 EER HIV-1 Genotype by Seq for Panel 11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HIV Genotype (Human Immunodeficiency Virus 1, Genotyping)
  • Raltegravir (Isentress{TM}) Resistance (HIV-1 Integrase Inhibitor Resistance by Sequencing)
HIV1 Genotype and Integrase Inhibitor Resistance by Sequencing