Pneumocystis jirovecii DFA with Reflex to Pneumocystis jirovecii by PCR (Test on Delay as of 04/29/22, temporarily unable to perform testing due to nationwide reagent backorder. ARUP recommends the following test: 2006254 Pneumocystis jirovecii by PCR)
Ordering Recommendation
Use for rapid identification of P. jirovecii. Negative DFA results are confirmed by PCR testing. Sensitivity is dependent on patient population and specimen type.
Mnemonic
Methodology
Direct Fluorescent Antibody Stain/Qualitative Polymerase Chain Reaction
Performed
Sun-Sat
Reported
1-6 days
New York DOH Approval Status
Specimen Required
Respiratory specimen: Bronchial washing, bronchoalveolar lavage (BAL), or induced sputum.
Transfer 5 mL respiratory specimen to a sterile container. (Min: 1 mL) Place each specimen in an individually sealed bag.
Refrigerated.
Tissues. Formalinized specimens. Slides or swabs.
Specimen source required.
Ambient: 2 hours; Refrigerated: 1 week; Frozen: Unacceptable
Reference Interval
Negative
Interpretive Data
FDA
Note
A negative stain result does not exclude the possibility of infection. False-negative results may occur due to sampling errors or a low number of organisms in the specimen. If Pneumocystis jirovecii DFA is negative, then Pneumocystis jirovecii by PCR testing will be added. Additional charges apply.
Hotline History
CPT Codes
87015; 87281; if reflexed, add 87798
Components
Component Test Code* | Component Chart Name | LOINC |
---|---|---|
2002588 | Pneumocystis source: | 31208-2 |
2009227 | Pneumocystis jirovecii - DFA | 700-5 |
Aliases
- Pneumocystis carinii