ROS1 with Interpretation by Immunohistochemistry with Reflex to FISH if Equivocal or Positive
Determine eligibility for tyrosine kinase inhibitor (TKI) (eg, crizotinib) therapy, primarily in individuals with pulmonary adenocarcinomas. Detects ROS1 fusion proteins (IHC) and ROS1 gene rearrangements (FISH).
1-5 days, add 3-5 days if reflexed
New York DOH Approval Status
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 6 unstained (3- to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect or contact ARUP Client Services at (800)522-2787. (Min: 6 slides) If sending precut slides, do not oven bake.
Room temperature or refrigerated. Ship in cooled container during summer months.
Paraffin block with no tumor tissue remaining. Specimens fixed in any other fixative other than 10 percent neutral buffered formalin. Cytology specimens fixed in alcohol. Decalcified specimens.
Include pathology report. IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services at (800) 522-2787.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Refer to report.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
If ROS1by Immunohistochemistry result is equivocal or positive, then ROS1 by FISH will be added. Additional charges apply.
88342; if reflexed, add 88366
|Component Test Code*||Component Chart Name||LOINC|
|2008415||ROS1 by IHC Result|
|2008417||ROS1 Client Block ID|
|3003121||ROS1 Tissue Source|
- ROS1 IHC
- ROS1 IHC with Interpretation
- ROS1 immunostaining