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Time Sensitive

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Cytogenetic Test Request Form Recommended (ARUP form #43098)
Ordering Recommendation

Use to test amniotic fluid for the following indications:
Abnormal US findings
Abnormal prenatal screen
Fetal demise
Genomic microarray testing is performed on normal chromosome analysis results.  In cases with a normal karyotype, microarray studies reveal clinically relevant copy number variations (CNV) in ~6% of fetuses with an ultrasound anomaly and ~2% whose indication is advanced maternal age or positive aneuploidy screen.

Mnemonic
AF REFLEX
Methodology

Giemsa Band/Genomic Microarray (Oligo-SNP Array)

Performed

Sun-Sat

Reported

7-14 days
If reflexed: 5-7 additional days required for microarray

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation

N/A

Collect

Amniotic fluid.

Specimen Preparation

Do not freeze or expose to extreme temperatures. Transport 15-30 mL amniotic fluid in a sterile container.

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

N/A

Remarks
Stability

Ambient: 48 hours; Refrigerated: 48 hours Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Refer to report

Compliance Category

Laboratory Developed Test (LDT)

Note

If Chromosome Analysis is "normal" or "no growth,"  then Genomic Microarray testing will be added.  Additional charges apply.

Maternal cell contamination studies recommended if testing reflexes to the fetal microarray. For maternal cell contamination studies if testing reflexes to the fetal microarray, please submit a maternal blood sample and order Microarray Genomic, Maternal Confirm (ARUP test code 2002369) accompanied by a test request form for the mother (this test is performed at no charge as part of the fetal microarray). Maternal blood should be submitted along with the amniotic fluid sample at the time the test ordered. For questions regarding ordering please contact ARUP's genetic counselor at (800) 242-2787 ext. 2141.

The chromosome analysis studies involve culturing of living cells; therefore, turnaround times given represent average times, which are subject to multiple variables.

A processing fee will be charged if this procedure is canceled at the client's request after the test has been set up or if the specimen integrity is inadequate to allow culture growth

This test must be ordered using Cytogenetic test request form 43098 or through your ARUP interface. Please submit the Patient History for Prenatal Cytogenetics form with the electronic packing list (https://ltd.aruplab.com/Tests/Pdf/65). Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.

Hotline History
N/A
CPT Codes

88269; 88235; 88291; if reflexed, add 81229; 81265

Components
Component Test Code* Component Chart Name LOINC
0097601 Chromosome Analysis, Amniotic Fluid 33773-3
2009476 EER Chrom Analysis AF w/Rflx to Array
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Amniotic Fluid, Chromosome Analysis
  • Array CGH, Prenatal
  • comparative genomic hybridization, prenatal
  • Karyotype, Amniotic Fluid
  • prenatal
  • Prenatal array
  • Prenatal Chromosomes, Amniotic Fluid
  • Prenatal Diagnosis
Chromosome Analysis, Amniotic Fluid, with Reflex to Genomic Microarray