Ordering Recommendation

Use to obtain quantitative results or to follow up a presumptive result. For general screening, the preferred test is Benzodiazepines Urine Screen with Reflex to Quantitation (2012225).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Random urine.

Specimen Preparation

Transfer 0.5 mL urine with no additives or preservatives to an ARUP standard transport tube. (Min: 0.3 mL)

Storage/Transport Temperature

Room temperature.

Unacceptable Conditions

Specimens exposed to repeated freeze/thaw cycles.


Ambient: 1 week; Refrigerated: 1 month; Frozen: 3 years


Quantitative Liquid Chromatography-Tandem Mass Spectrometry




1-5 days

Reference Interval

Drugs Covered Cutoff Concentrations
Alprazolam 5 ng/mL
Alpha-hydroxyalprazolam 5 ng/mL
Chlordiazepoxide 20 ng/mL
Clonazepam 5 ng/mL
7-aminoclonazepam 5 ng/mL
Diazepam 20 ng/mL
Lorazepam 20 ng/mL
Midazolam 20 ng/mL
Alpha-hydroxymidazolam 20 ng/mL
Nordiazepam 20 ng/mL
Oxazepam 20 ng/mL
Temazepam 20 ng/mL

Interpretive Data

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry

Drugs covered:
alprazolam, alpha-hydroxyalprazolam, chlordiazepoxide, clonazepam, 7-aminoclonazepam, diazepam, lorazepam, midazolam, alpha-hydroxymidazolam, nordiazepam, oxazepam and temazepam.. 

Positive cutoff:
20 ng/mL unless specified below:
Alprazolam: 5 ng/mL
Alpha-hydroxyalprazolam: 5 ng/mL
Clonazepam: 5 ng/mL
7-aminoclonazepam: 5 ng/mL

For medical purposes only; not valid for forensic use.

Identification of specific drug(s) taken by specimen donor is problematic due to common metabolites, some of which are prescription drugs themselves. The absence of expected drug(s) and/or drug metabolite(s) may indicate noncompliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

Compliance Category

Laboratory Developed Test (LDT)


Compare to Pain Management, Benzodiazepines, Quantitative, w/ medMATCH, Urine (Drugs of Abuse Confirmation/Quantitation - Benzodiazepines - Urine); Pain Management, Benzodiazepines, w/Confirmation w/med MATACH, Urine (Drugs of Abuse Confirmation/Quantitation - Benzodiazepines - Urine).

Hotline History


CPT Codes

80346 (Alt code: G0480)


Component Test Code* Component Chart Name LOINC
2007834 Diazepam, Urn, Quant 16227-1
2007835 Oxazepam, Urn, Quant 16201-6
2007836 Temazepam, Urn, Quant 20559-1
2007837 Nordiazepam, Urn, Quant 16228-9
2007838 Lorazepam, Urn, Quant 17088-6
2007839 Alprazolam, Urn, Quant 59615-5
2007840 Alpha-hydroxyalprazolam, Urn, Quant 16348-5
2007841 Clonazepam, Urn, Quant 16229-7
2007842 7-aminoclonazepam, Urn, Quant 51776-3
2007846 Midazolam, Urn, Quant 16233-9
2007847 Chlordiazepoxide, Urn, Quant 20522-9
2008511 Alpha-hydroxymidazolam, Urn, Quant 59590-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Benzodiazepines, Urine, Quantitative