Chimerism, Additional Donor

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Ordering Recommendation

Assess donor genotype.

Mnemonic

STR-AD DON

Methodology

Polymerase Chain Reaction/Fragment Analysis

Performed

Sun-Sat

Reported

5-9 days

New York DOH Approval Status

This test is New York DOH approved.

Specimen Required

Patient Preparation

Post-transplantation results will be compared to pre-transplant recipient and donor genotypes, therefore, donor and recipient samples must be obtained and genotyped before the transplant event occurs.

Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). OR Bone marrow in lavender (EDTA). OR Buccal brushes from donor.

Specimen Preparation

Transport 3 mL whole blood. (Min: 1 mL) OR Transport 1 mL bone marrow. (Min: 1 mL) OR Transport 2 buccal brushes in a sterile, dry tube. (Min: 2 brushes)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions
Remarks
Stability

Ambient: 1 week; Refrigerated: 1 month; Frozen: 6 months

Reference Interval

By report

Interpretive Data

Background Information for Chimerism, Donor:
Indication: Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used: AmpFLSTR Identifiler PCR Amplification Kit, Applied Biosystems.
Limit of Detection: 2 percent of minor cell population. Diagnostic errors can occur due to rare sequence variations.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

81266; each additional donor

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Components

Component Test Code* Component Chart Name LOINC
0050759 Chimerism, Donor, Interpretation
2001378 Chimerism, Donor, Specimen
2002031 Chimerism, Donor, Recipient's Name
2002032 Chimerism, Donor, Informative Loci
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Bone marrow DNA typing
  • Chimerism evaluation
  • Pre transplant evaluation
  • Transplant analysis
  • Transplant evaluation
Chimerism, Additional Donor