Ordering Recommendation

Order to confirm a reactive treponemal screening test (eg, CIA, EIA) when using the reverse testing algorithm to screen for syphilis.

Mnemonic
RPR REV
Methodology

Semi-Quantitative Charcoal Agglutination/Semi-Quantitative Particle Agglutination

Performed

Sun-Sat

Reported

1-4 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube (SST).

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.4 mL).  Avoid freezing if possible.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, CSF, or other body fluids.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
0050471 Rapid Plasma Reagin (RPR) with Reflex to Titer
Test Code Components Reference Interval
Rapid Plasma Reagin (RPR) RPR (+) = Reactive
RPR (-) = Nonreactive
Rapid Plasma Reagin (RPR) Titer < 1:1

0050777 Treponema pallidum Antibody by TP-PA Nonreactive

Interpretive Data



Compliance Category

FDA

Note

If RPR is reactive, then a titer to endpoint will be added. If RPR is nonreactive, a TP-PA (MHA) confirmation will be added. Additional charges apply.

Hotline History
N/A
CPT Codes

86592 RPR; if reflexed, add (nonreactive) TP-PA 86780 or (reactive) 86593 RPR titer

Components
Component Test Code* Component Chart Name LOINC
0050472 Rapid Plasma Reagin (RPR) 20507-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Rapid Plasma Reagin (RPR) with MHA-TP Confirmation
  • Rapid Plasma Reagin (RPR), Reverse Algorithm, Syphilis Reverse Algorithm
  • Rapid Plasma Reagin Reflex to Titer or TPPA
  • RPR (Diagnosis) with Reflex to Titer and Confirmatory Testing
  • RPR Reflex to TPPA or Titer
  • RPR Reverse Algorithm
  • RPR with MHA-TP Confirmation
  • Syphilis Reverse Testing Algorithm
  • Treponema pallidum (Rapid Plasma Reagin) with Reflex to Titer & TP-PA Confirmation
Rapid Plasma Reagin (RPR) with Reflex to RPR Titer or T. pallidum Antibody by Particle Agglutination