Ordering Recommendation

Order to confirm a reactive treponemal screening test (eg, CIA, EIA) when using the reverse testing algorithm to screen for syphilis.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Serum separator tube (SST).

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP standard transport tube. (Min: 0.5 mL).  Avoid freezing if possible.

Storage/Transport Temperature


Unacceptable Conditions

Contaminated, grossly hemolyzed, grossly lipemic, plasma, CSF, or other body fluids.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)


Semi-Quantitative Particle Agglutination




1-4 days

Reference Interval

Test Number
Reference Interval
  Rapid Plasma Reagin (RPR) Non Reactive

Interpretive Data

Component Interpretation
Rapid Plasma Reagin (RPR) RPR (+) = Reactive
RPR (-) = Nonreactive

Compliance Category



If RPR is reactive, then a titer to endpoint will be added. If RPR is nonreactive, a TP-PA (MHA) confirmation will be added. Additional charges apply.

Hotline History


CPT Codes

86592 RPR; if reflexed, add (nonreactive) TP-PA 86780 or (reactive) 86593 RPR titer


Component Test Code* Component Chart Name LOINC
0050472 Rapid Plasma Reagin (RPR) 20507-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Rapid Plasma Reagin (RPR) with MHA-TP Confirmation
  • Rapid Plasma Reagin (RPR), Reverse Algorithm, Syphilis Reverse Algorithm
  • Rapid Plasma Reagin Reflex to Titer or TPPA
  • RPR (Diagnosis) with Reflex to Titer and Confirmatory Testing
  • RPR Reflex to TPPA or Titer
  • RPR Reverse Algorithm
  • RPR with MHA-TP Confirmation
  • Syphilis Reverse Testing Algorithm
  • Treponema pallidum (Rapid Plasma Reagin) with Reflex to Titer & TP-PA Confirmation
Rapid Plasma Reagin (RPR) with Reflex to RPR Titer or T. pallidum Antibody by Particle Agglutination