Ordering Recommendation

Confirm a diagnosis of hairy cell leukemia (HCL) and monitor tumor burden.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Whole blood or bone marrow in lavender (EDTA).

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or frozen tissue. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.

Remarks
Stability

Refrigerated: 7 days; Frozen: Unacceptable

Methodology

Polymerase Chain Reaction (PCR)

Performed

Varies

Reported

4-10 days

Reference Interval

Interpretive Data

Refer to report.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

81210

Components

Component Test Code* Component Chart Name LOINC
2007133 BRAF V600E Detection Hairy Cell, Result 85101-4
2007134 BRAF V600E Detection Hairy Cell, Quant 81258-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • BRAF V600E
  • Hairy cell leukemia BRAF testing
  • Hairy cell leukemia quant PCR
BRAF V600E Mutation Detection in Hairy Cell Leukemia by Real-Time PCR, Quantitative