Ordering Recommendation

Confirm a diagnosis of hairy cell leukemia (HCL) and monitor tumor burden.

Mnemonic
BRAF HCL
Methodology

Polymerase Chain Reaction

Performed

DNA isolation: Sun-Sat
Testing: Varies

Reported

4-10 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA) or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA certified lab.

Specimen Preparation

Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.

Remarks
Stability

Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely

Reference Interval
Interpretive Data

Refer to report

Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

81210

Components
Component Test Code* Component Chart Name LOINC
2007133 BRAF V600E Detection Hairy Cell, Result
2007134 BRAF V600E Detection Hairy Cell, Quant
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Aliases
  • BRAF V600E
  • Hairy cell leukemia BRAF testing
  • Hairy cell leukemia quant PCR
BRAF V600E Mutation Detection in Hairy Cell Leukemia by Real-Time PCR, Quantitative