BRAF V600E Mutation Detection in Hairy Cell Leukemia by Real-Time PCR, Quantitative
Confirm a diagnosis of hairy cell leukemia (HCL) and monitor tumor burden.
Polymerase Chain Reaction
DNA isolation: Sun-Sat
Lavender (EDTA) or bone marrow (EDTA). Also acceptable: DNA extracted by CLIA certified lab.
Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Transport 3 mL bone marrow. (Min: 1 mL)
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely
Refer to report
Laboratory Developed Test (LDT)
|Component Test Code*||Component Chart Name||LOINC|
|2007133||BRAF V600E Detection Hairy Cell, Result|
|2007134||BRAF V600E Detection Hairy Cell, Quant|
- BRAF V600E
- Hairy cell leukemia BRAF testing
- Hairy cell leukemia quant PCR