May be useful in immunization/vaccination trials or may aid in the clinical diagnosis of diseases strongly associated with the HLA-C locus.
Polymerase Chain Reaction/Sequence-Specific Oligonucleotide Probe Hybridization
New York DOH Approval Status
Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B).
Transport 5 mL whole blood. (Min: 3 mL)
Specimens collected in green (sodium or lithium heparin) tubes.
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Background Information for HLA-C Genotype:
Purpose: For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-C locus.
Methodology: PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-C locus.
Analytical Sensitivity & Specificity: Medium to high resolution of the HLA-C locus.
Limitations: The presence of a disease-associated HLA combination does not establish a diagnosis. If fewer than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation; for pre-transplant allele matching, consider HLA Class I and II Panel (HLA A, HLA B, HLA C, DRB1, DQA1, DQB1, DPB1) by Next Generation Sequencing (ARUP test code 3002061) or HLA Class I and II Panel (HLA A, HLA B, HLA C, DRB1, DRB345, DQA1, DQB1, DPA1, DPB1) by Next Generation Sequencing (ARUP test code 3002062).
Occasionally the specific allele cannot be determined; in this case, the most likely allele assignment is made followed by a sequence of letters indicating other possible allele assignments. Interpretation of allele codes can be found at https://bioinformatics.bethematchclinical.org/hla/alpha.v3.html.
Test systems were developed and their performance characteristics determined by the H&I laboratory at the University of Utah Health, under the accreditation guidelines from the American Society for Histocompatibility and Immunogenetics (ASHI).
Performed by non-ARUP Laboratory
Order this test for single antigen HLA-C identification. Please specify antigens.
|Component Test Code*||Component Chart Name||LOINC|
|2002808||HLA Class I, Locus C, Allele 1||42663-5|
|2002809||HLA Class I, Locus C, Allele 2||42663-5|
|2006989||HLA-C Genotype Interpretation|