Ordering Recommendation

May be useful in immunization/vaccination trials or may aid in the clinical diagnosis of diseases strongly associated with the HLA-B locus.

Mnemonic
HLA B
Methodology

Polymerase Chain Reaction/Sequence Specific Oligonucleotide Probe Hybridization

Performed

Mon-Fri

Reported

3-7 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).

Specimen Preparation

Transport 5 mL whole blood. (Min: 3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Specimens collected in green (sodium or lithium heparin) tubes.

Remarks
Stability

Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable

Reference Interval

By report

Interpretive Data

Background Information for HLA-B Genotype:
Purpose:
For immunization/vaccination trials or to aid the clinical diagnosis of diseases strongly associated with the HLA-B locus.
Methodology:
PCR followed by Sequence Specific Oligonucleotide Probe Hybridization of HLA-B locus.
Analytical Sensitivity & Specificity:
Medium to high resolution of HLA-B locus.
Limitations:
The presence of a disease-associated HLA combination does not establish a diagnosis. If fewer than 2 alleles are reported for a locus, the patient is likely homozygous. Rare diagnostic errors can occur due to primer or probe site mutations. This test is not sufficient for comprehensive HLA evaluation for clinical hematopoietic stem cell transplantation; for pre-transplant allele matching, consider HLA Class I (ABC) by Next Generation Sequencing - (ARUP test code 2011264) and/or HLA Class II (DRB1 and DQB1) by Next Generation Sequencing (ARUP test code 2011272).

Occasionally the specific allele cannot be determined; in this case, the most likely allele assignment is made followed by a sequence of letters indicating other possible allele assignments. Interpretation of allele codes can be found at https://bioinformatics.nmdp.org/HLA/alpha.v3.html.

Counseling and informed consent are recommended for genetic testing. Consent forms are available online at www.aruplab.com.

Compliance Statement C: For human genetic inheritable conditions and mutations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Counseling and informed consent are recommended for genetic testing. Consent forms are available online.

Note

Order this test for single antigen HLA-B identification. Please specify antigens.

Hotline History
N/A
CPT Codes

81380

Components
Component Test Code* Component Chart Name LOINC
2002785 HLA Class I, Locus B*, Allele 1 13299-3
2002786 HLA Class I, Locus B*, Allele 2 13299-3
2006987 HLA-B Genotype Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
HLA-B Genotype