Ordering Recommendation

Use following an abnormal amniotic fluid alpha fetoprotein (AFP) result to evaluate possibility of a fetal open neural tube defect.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Amniotic Fluid

Specimen Preparation

Transfer 2 mL amniotic fluid to an ARUP Standard Transport Tube. (Min: 1 mL)

Storage/Transport Temperature


Unacceptable Conditions

Include the Amniotic Fluid AFP and MoM results, if available.


Ambient: 2 months; Refrigerated: 4 months; Frozen: 3 years


Qualitative Gel Electrophoresis/Radial Immunodiffusion


Mon, Wed


3-11 days

Reference Interval

Acetylcholinesterase: Negative
Fetal Hemoglobin: Negative

Interpretive Data

Refer to report

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes

82013; 83033


Component Test Code* Component Chart Name LOINC
2006849 Acetylcholinesterase, Amniotic Fluid 30106-9
2006850 Fetal Hemoglobin, Amniotic Fluid 28067-7
2006853 Acetylcholinesterase/Fetal HGB Interp 48767-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Acetylcholinesterase Amniotic Fluid AFP
  • Acetylcholinesterase, AF
  • Acetylcholinesterase, Amniotic Fluid
  • AChE-AF
  • Amniotic Fluid, Acetylcholinesterase
  • Fetal Hemoglobin, Amniotic Fluid
Acetylcholinesterase and Fetal Hemoglobin, Amniotic Fluid