Ordering Recommendation
Mnemonic
HIV AGAB
Methodology

Qualitative Chemiluminescent Immunoassay /Qualitative Western Blot

Performed

Sun-Sat

Reported

1-2 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect

Serum separator tube (SST). Also acceptable: Lavender (EDTA), pink (K2EDTA).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1.5 mL serum or plasma to an ARUP Standard Transport Tube. Remove particulate material. (Min: 0.75 mL) Remove particulate material.

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Specimens containing particulate material. Severely hemolyzed or heat-inactivated specimens.

Remarks
Stability

After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 8 months (avoid repeated freeze/thaw cycles)

Reference Interval
Test Number
Components
Reference Interval
Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by CIA Negative
0020284 Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot Negative

Interpretive Data

This test should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

Compliance Category

FDA

Note

This fourth-generation screen test is for the simultaneous qualitative detection of Human Immunodeficiency Virus Type 1 (HIV-1) p24 antigen and antibodies to HIV Type 1 (HIV-1 groups M and O) and HIV Type 2 (HIV-2), Results of the screen cannot be used to distinguish between the presence of HIV-1 p24 antigen, HIV-1 antibody, or HIV-2 antibody.

If the HIV-1,2 Combo Antigen/Antibodies screen is repeatedly reactive, then the Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot will be added. Additional charges apply.

Performed and Reported times indicated are for the screening portion of this test. Refer to Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western Blot (ARUP test code 0020284) for additional information regarding Performed or Reported times for the reflex portion of this test.

Hotline History
N/A
CPT Codes

87389; if reflexed, add 86689

Components
Component Test Code* Component Chart Name LOINC
2006611 HIV-1,2 Combo Antigen/Antibody 56888-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 4th generation HIV screen; HIV Ag/Ab combination screen
  • Fourth generation HIV
  • HIV 1,2 Combo Antigen/Antibody
  • HIV Diagnosis
  • HIV-1,2 Combo Antigen/Antibody
Human Immunodeficiency Virus (HIV) Combo Antigen/Antibody (HIV-1/O/2) by CIA, with Reflex to HIV-1 Antibody Confirmation by Western Blot