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B-Cell Clonality Screening (IgH and IgK) by PCR
Copy Utility
Ordering Recommendation
Aids in the diagnosis and monitoring of B-cell lymphoproliferative disorders. This is an ancillary test that may be used in the context of additional morphologic and clinical evaluation to differentiate between malignant and reactive proliferations. This test uses primers from the BIOMED-2 primer set that target IgH (VHFR1-JH; VHFR2-JH; VHFR3-JH) and IgK (VK-JK; VK-Kde; JKCK intron-Kde) genes.
Mnemonic
Methodology
Capillary Electrophoresis
Performed
Varies
Reported
5-9 days
New York DOH Approval Status
Specimen Required
Whole blood or bone marrow (EDTA), tissue, formalin-fixed tissue.
Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
Fresh Tissue: Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue.
FFPE Tumor Tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Tissue block will be returned after testing. Transport tissue block or four 10-micron shavings in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Whole Blood, Bone Marrow: Refrigerated.
Fresh Tissue: Frozen on dry ice.
FFPE Tumor Tissue: Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months
Plasma, serum. Specimens collected in anticoagulants other than EDTA. Clotted or grossly hemolyzed specimens.
Tissue: Specimens fixed/processed in alternative fixatives, heavy metal fixatives (B-4 or B-5), or tissue sections on slides. Decalcified specimens.
If multiple specimens (blocks or slides) are sent to ARUP, they must be accompanied by one of the following: an order comment indicating that the ARUP pathologist should choose the specimen most appropriate for testing (e.g., "Choose best block"), or individual orders for each sample submitted. A Pathologist Block Selection Fee (ARUP test code 3002076) will be added to orders that utilize the first option. If multiple specimens are sent to ARUP without a request for pathologist block/slide selection or individual orders, they will be held until clarification is provided.
Whole Blood or Bone Marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Fresh Tissue: Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
FFPE Tumor Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely
Reference Interval
Interpretive Data
Refer to report.
This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Laboratory Developed Test (LDT)
Note
Hotline History
Hotline History
CPT Codes
81261; 81264
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 2006287 | B-Cell Clonality, Source | 31208-2 |
| 2006288 | B-Cell Clonality, IgH and IgK by PCR |
Aliases
- B-cell clonality screening
- IgH and IgK B-cell clonality
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