Useful to ensure the highest chance of obtaining meaningful results from fetal specimens. When tissue culture is unsuccessful or if the results of the chromosome analysis are normal, then testing reflexes to genomic microarray.
Giemsa Band/Genomic Microarray (Oligo-SNP Array)
If reflexed: 10-15 additional days are required for microarray
Thaw media prior to tissue inoculation. Products of conception in a sterile, screw-top container (Wide mouth containers: ARUP supply #42710) filled with tissue culture transport medium (ARUP Supply #32788). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. If cytogenetics tissue media is not available, collect in plain RPMI, Hanks solution, sterile saline, or ringers.
If autopsy is performed: Facia lata, diaphragm, tendon, skin, tissue from internal organs (if fresh), chest wall cartilage (particularly if macerated) or placenta from fetal side.
If no autopsy is performed: Placenta from fetal side is preferred (e.g. villi).
Also acceptable: Umbilical cord or Achilles tendon.
DO NOT FREEZE. Do not place in formalin. Transport products of conception (min: 5mg) in sterile, screw-top container filled with tissue transport medium. If specimen size is too large for a normal collection tube, a larger sterile container can be used such as a sterile urine cup and can be flooded with several tubes of cytogenetic tissue media.
Room temperature (fresh tissue or culture flask). Also acceptable: Refrigerated.
Frozen specimens. Intact fetus. Specimens preserved in formalin. Specimens consisting of maternal tissue (decidua) only. Autolyzed or contaminated specimens
If specimen collection time is greater than 72 hours, testing may be compromised. The laboratory will make every attempt to culture the specimen. Send specimen to lab for testing.
Ambient: 48 hours; Refrigerated:48 hours; Frozen: Unacceptable
Refer to report
Laboratory Developed Test (LDT)
The chromosome analysis studies involve culturing of living cells; therefore, turnaround times given represent average times, which are subject to multiple variables.
A processing fee will be charged if the client cancels this procedure after the test has been set up or if the specimen integrity is inadequate to allow culture growth.
When the result of Chromosome Analysis is either "no growth" or "normal," then Genomic Microarray testing will be added. Additional charges apply.
This test must be ordered using Cytogenetic test request form #43098 or through your ARUP interface. Please submit the Patient History for Prenatal Cytogenetics form with the electronic packing list (https://ltd.aruplab.com/Tests/Pdf/65).
88262; 88233; 88291; If reflexed, add 81229
|Component Test Code*||Component Chart Name||LOINC|
|0097645||Chromosome Analysis, Prod Concp||48819-7|
|2006187||EER Chrom Analysis POC w/Rflx to Array|
- Array Comparative Genomic Hybridization
- Autopsy (ONLY if Chromosomes are indicated)
- Chromosomal Microarray
- Chromosome Analysis with reflex to microarray, Stillbirth or Miscarriage
- Chromosome Analysis, Stillbirth or Miscarriage
- comparative genomic hybridization
- Congenital Array
- Constitutional Array
- intrauterine fetal demise
- Karyotype, Stillbirth or Miscarriage
- missed abortion
- Molecular Karyotype
- Oligo Array
- Oligonucleotide Array
- Pregnancy loss
- Single-nucleotide-polymorphism (SNP) array
- Spontaneous abortion
- Whole Genome Array