Semi-Quantitative Ex Vivo Challenge/Cell Culture/Quantitative Enzyme-Linked Immunosorbent Assay/Quantitative Chemiluminescent Immunoassay
Patients taking calcineurin inhibitors should stop their medication 72 hours prior to draw. Patients on prednisone should be off their medication for 2 weeks prior to draw.
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport tube and freeze immediately (Min: 0.5 mL) AND transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)
First Specimen: CRITICAL FROZEN. Separate specimens must be submitted for this multiple test panel.
Second Specimen: Refrigerated.
Specimens other than serum. Contaminated, grossly hemolyzed, or lipemic specimens.
First Specimen: After separation from cells: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Second Specimen: After separation from cells: Ambient: 8 hours; Refrigerated: 1 Week; Frozen: 6 months
Refer to report.
Laboratory Developed Test (LDT)
1) Chronic urticaria (CU) is a common and complex dermatological condition that is suspected when patients experience persistent hives for over 6 weeks. No published evidence of an exogenous allergen as the cause of this disorder exists. About 45 percent of cases have autoantibodies directed against either basophil or mast cell-associated IgE or the high affinity IgE-Fc receptor (Fc epsilon R1 alpha) (Clin Exp Allergy 2009; 39: 777-87
2) The presence of histamine-releasing factors (including but not limited to IgE and Fc epsilon R1 alpha-specific autoantibodies) in the patient serum can be indirectly determined by evaluating basophil/mast cell activation status using histamine-release assays, autologous serum-skin test, and flow cytometric measurement of the basophil and mast cell-specific marker CD203c. Serum from CU patients can activate donor basophils, which induces histamine release and CD203c upregulation (J Allergy Clin Immunol 2006; 117: 1430-4).
86352; 86800; 84443; 86376
|Component Test Code*||Component Chart Name||LOINC|
|0050075||Thyroid Peroxidase (TPO) Antibody||8099-4|
|0070145||Thyroid Stimulating Hormone||3016-3|
- Chronic Urticaria
- Chronic Urticaria Panel
- urticaria-inducing activity, TPO, TSH, Tg antibodies