Ordering Recommendation

In most cases, serology testing is not recommended for the diagnosis of active pertussis infection. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).

Mnemonic
BORDIGG
Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Tue, Fri

Reported

1-5 days

New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Specimen Required
Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Effective February 20, 2018
0.94 IV or less Negative - No significant level of detectable B. pertussis IgG antibody.
0.95-1.04 IV Equivocal Repeat testing in 10-14 days may be helpful.
1.05 IV or greater Positive IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.

Interpretive Data



Compliance Category

Laboratory Developed Test (LDT)

Note
Hotline History
N/A
CPT Codes

86615

Components
Component Test Code* Component Chart Name LOINC
2001782 B. pertussis Ab, IgG by ELISA 42330-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • B. pertussis IgG ELISA
  • Pertussis Antibody
  • Pertussis IgG Ab ELISA
Bordetella pertussis Antibody, IgG by ELISA