Ordering Recommendation

In most cases, serology testing is not recommended for the diagnosis of active pertussis infection. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).

Mnemonic

BORDIGG

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Tue, Fri

Reported

1-5 days

New York DOH Approval Status

Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval

Effective February 20, 2018
0.94 IV or less Negative - No significant level of detectable B. pertussis IgG antibody.
0.95-1.04 IV Equivocal Repeat testing in 10-14 days may be helpful.
1.05 IV or greater Positive IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

86615

Components

Component Test Code* Component Chart Name LOINC
2001782 B. pertussis Ab, IgG by ELISA 42330-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • B. pertussis IgG ELISA
  • Pertussis Antibody
  • Pertussis IgG Ab ELISA
Bordetella pertussis Antibody, IgG by ELISA