Ordering Recommendation

Useful as an indicator of in vivo breakdown of hemoglobin to differentiate cerebral hemorrhage from a traumatic tap.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

CSF. Collect at least 12 hours after suspected hemorrhage. Use the last (ideally the fourth) specimen post lumbar puncture. AND serum separator tube.

Specimen Preparation

CSF: Transport to local lab quickly and avoid pneumatic tube transport if possible. Protect from light during collection, storage and shipment. Centrifuge for 5 minutes ASAP or within 1 hour of collection. Transfer 2 mL CSF to an ARUP Amber Transport Tube. (Min: 1 mL) Do not freeze.
Serum: Protect from light during collection, storage and shipment. Allow specimen to clot completely at room temperature. Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Amber Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

Specimens not protected from light. Frozen CSF.


CSF: Ambient: 4 hours; Refrigerated: 1 week; Frozen: Unacceptable
Serum: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months


Quantitative Spectrophotometry




Within 24 hours

Reference Interval

Test Number
Reference Interval
  Net Absorbance, Bilirubin, CSF 0.007 AU or less
  Net Absorbance, Oxyhemoglobin, CSF 0.020 AU or less
  Serum Bilirubin Refer to Report

Interpretive Data

Refer to report.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes

84311 x2; 82247


Component Test Code* Component Chart Name LOINC
2005249 Net Absorbance, Bilirubin, CSF
2005250 Net Absorbance, Oxyhemoglobin, CSF
2005252 Serum Bilirubin 1975-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • Xanthochromia
Bilirubin, CSF