Do not use in the initial evaluation for celiac disease. Useful in ruling out celiac disease in selective clinical situations, such as equivocal small bowel histologic finding or individuals on a gluten-free diet not tested for celiac disease prior to starting the diet.
Polymerase Chain Reaction/Fluorescence Monitoring
Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B).
Transport 3 mL whole blood. (Min: 1 mL)
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
Background Information for Celiac Disease (HLA-DQ2, and HLA-DQ8) Genotyping:
Characteristics: Celiac disease is a systemic autoimmune disorder that may be associated with gastrointestinal symptoms including: diarrhea, weight loss, anorexia, lactose intolerance, and abdominal distention and discomfort. Non-gastrointestinal characteristics are highly variable and include: chronic fatigue, joint pain/inflammation, migraines, epilepsy, depression, attention deficit disorder, iron-deficiency anemia, vitamin deficiency, osteoporosis/osteopenia, short stature, delayed puberty, dental enamel defects, infertility, recurrent fetal loss, and dermatitis herpetiformis.
Incidence: One in 133 individuals in the US is affected.
Cause: The presence of either the HLA-DQ2 or the HLA-DQ8 allele in combination with dietary gluten.
Alleles tested: HLA-DQ2 (encoded by HLA-DQA1*05 and HLA-DQB1*02) and HLA-DQ8 (encoded by HLA-DQB1*03:02).
Clinical Sensitivity and Specificity: Approximately 100 percent and 3 percent, respectively.
Methodology: PCR with melting curve analysis.
Analytical Sensitivity and Specificity: 99 percent.
Limitations: Rare diagnostic errors may occur due to primer site mutations. Copy number of each detected allele will not be determined. Alleles other than HLA-DQ2 and HLA-DQ8 will not be identified. Other genetic and non-genetic factors that influence celiac disease are not evaluated.
Laboratory Developed Test (LDT)
81383; 81376 x2
|Component Test Code*||Component Chart Name||LOINC|
|2005022||Celiac HLA Interpretation||48767-8|
|2011755||HLA Celiac Specimen||31208-2|
- Celiac Disease genotyping
- HLA Celiac Disease Testing
- HLA DQ
- HLA genotyping for Celiac Disease
- HLA-DQ; HLA DQ
- HLA-DQA1*05, HLA-DQB1*02, & *03