Ordering Recommendation

Evaluation of IgM pertussis antibodies has little clinical utility. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).

New York DOH Approval Status

This test is not New York state approved. There are no New York state-approved laboratories available. Submit a Non-Permitted Laboratory Request Form (NPL) to the NYDOH prior to collection of specimen. If NPL is approved by NYDOH, and sample is received at ARUP, testing will be performed.

Specimen Required

Patient Preparation

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)

Storage/Transport Temperature


Unacceptable Conditions

Heat-inactivated specimens.


After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year


Qualitative Immunoblot


Tue, Fri


1-5 days

Reference Interval

Effective February 19, 2013

Bordetella pertussis Antibody, IgM by Immunoblot
Reference Interval
Bordetella pertussis Ab, IgM by Immunoblot Interp Negative
B. pertussis, IgM Immunoblot PT Negative
B. pertussis, IgM Immunoblot FHA Negative

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


This assay tests for the presence of pertussis toxin (PT) and filamentous hemagglutinin antibody (FHA).

Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
2004323 B. pertussis Ab, IgM Immunoblot Interp 93479-4
2004324 B. pertussis, IgM Immunoblot FHA 43882-0
2004325 B. pertussis, IgM Immunoblot PT 69368-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


  • B. pertussis IgM immunoblot
  • Pertussis Antibody
  • Pertussis IgM Ab Immunoblot
Bordetella pertussis Antibody, IgM by Immunoblot