Ordering Recommendation

Aids in management of individuals treated with interferon beta.

Mnemonic
IFNB NEU R
Methodology

Cell Culture/Chemiluminescent Immunoassay

Performed

Mon

Reported

1-15 days

New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation

Collect specimens before Interferon beta treatment, or more than 48 hours following the most recent dose. Patient should not be on steroid therapy in excess of 10 mg prednisolone (or equivalent) daily. High endogenous levels of Interferon beta, alpha, or gamma may interfere with this assay.

Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Contaminated, hemolyzed, icteric, or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Reference Interval
Available Separately
Components
Reference Interval
Interferon Beta Screen Negative
Interferon Beta Titer Less than 20: Negative
20 to 99: Moderate levels of neutralizing antibodies present
100 or greater: High levels of neutralizing antibodies present

Interpretive Data

A titer of 20 to 99 suggests moderate levels of neutralizing antibodies present. A titer of 100 or greater suggests high levels of neutralizing antibodies present. Either case warrants continued patient monitoring. The titer is reported as the reciprocal of the dilution of patient serum that reduces the interferon activity by 10 fold.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note

If Interferon Beta Neutralizing Antibody screen result is positive, then Interferon Beta Neutralizing Antibody titer will be added. 

This test is performed pursuant to an agreement with Biomonitor.

Hotline History
N/A
CPT Codes

86352; if reflexed, add 86352

Components
Component Test Code* Component Chart Name LOINC
2003391 Interferon Beta Neutralizing Ab Screen 29647-5
2003392 Interferon Beta Neutralizing Ab Titer 35139-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • iLite
  • Interferon-Beta
  • NAb
  • NabFeron
  • Neutralization
Interferon Beta Neutralizing Antibody with Reflex to Titer