Ordering Recommendation

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation

Specimen should be collected between 8-10 a.m.


Serum separator tube, lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation

Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)

Storage/Transport Temperature


Unacceptable Conditions

After separation from cells: Ambient: 1 week; Refrigerated: 1 week; Frozen: 6 months


Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry


Wed, Sat


2-5 days

Reference Interval

Adult Reference Ranges for Cortisol, Serum, µg/dL
AM 4.6-20.6 µg/dL
PM 1.8-13.6 µg/dL
Stimulation 30-60  Minutes Post 0.25 mg Cosyntropin IV Greater than 20.0 µg/dL

Pediatric Reference Ranges for Cortisol, Serum, µg/dL
1-12 months 4.6-23.0 µg/dL
1-5 years 6.0-25.0 µg/dL
6-17 years 4.6-15.0 µg/dL
Stimulation 30-60 Minutes Post 0.25 mg Cosyntropin IV
1-12 months 32.0-60.0 µg/dL
1-5 years 22.0-40.0 µg/dL
6-17 years 17.0-28.0 µg/dL

Interpretive Data

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)


Hotline History


CPT Codes



Component Test Code* Component Chart Name LOINC
2003251 Cortisol by LC-MS/MS, Serum or Plasma 2143-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.


Cortisol by LC-MS/MS, Serum or Plasma